The phrase
"mouse zygotes" refers to the earliest stage of development in a mouse embryo. It is when a sperm cell fertilizes an egg cell resulting in the formation of a single cell, which has the potential to grow into a full-grown mouse.
Full definition
The work, published today (October 19) in PNAS, shows that simultaneously injecting nine miRNAs
into mouse zygotes recapitulates the changes found in the offspring of stressed mice.
Efficient gene targeting of the Rosa26 locus
in mouse zygotes using TALE nucleases.
In a new study published today in the Proceedings of the National Academy of Sciences, researchers report that
mouse zygotes injected with miRNAs develop the same blunted cortisol response — demonstrating that the miRNAs are at least partially to blame.
Using the gene - editing tool CRISPR - Cas9 to turn off certain genes in
a mouse zygote as well as other new techniques to enrich the pluripotent stem cells of a rat, the group managed to grow various rat organs (a pancreas, heart, and eyes) in a mouse embryo.
and a gRNA in one AAV and a repair template in second a second AAV to deliver their repair template to
mouse zygotes and thereby correct a mutation that causes Hemophilia B.
Ohmori et al 2017 use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in second a second AAV to deliver their repair template to
mouse zygotes and thereby correct a mutation that causes Hemophilia B.
Alternatively, the gRNA, Cas9, and donor oligonucleotide can also be electroporated into
the mouse zygote -LRB-
Alternatively, the gRNA, Cas9, and donor oligonucleotide can also be electroporated into
the mouse zygote (Qin et al., 2015).