Sentences with phrase «negative control»

All therapies contain positive and negative controls on behavior.

It is not clear what an explanation for plate based methods would be, and no proper negative control data exist for plate based methods to investigate these properties.

Both mothers and fathers of children with borderline intellectual functioning have more negative controlling parenting (child age 5 - 6) than did parents of typically developing children.

Vero E6 cells without virus inoculation and HeLa cells without ACE2 were used as negative control.

The combination of the high structural similarity between UNC0737 and UNC0638 and the > 300-fold loss of potency in UNC0737 compared to UNC0638 makes UNC0737 an appropriate negative control for use in cellular and functional assays.

On a similar note, the latest single cell sequencing methods based on stochastic schemes for in situ barcoding of cells are impossible to assess with negative control samples.

Negative controls consisted of naïve C57BL / 6 mice that had been exposed to BALB / c alloantigens in vivo.

Each sample DNA was amplified by PCR with primers to the STAT6 binding site or negative control primers to ensure equal amounts of DNA were utilized in the PCR reaction.

Negative control experiments using the same primers on ganglia of 5 - HT3AKO mice show no detectable 5 - HT3A mRNA.

Negative control slides were immunostained with the same method in the absence of the primary antibody [Ab -LRB--)-RSB-.

Negative controls included Lycopersicum esculentum, Saccharomyces cerevisiae and derivatized blanks.

Cells were transfected with 320 ng pGL3 luciferase expression construct containing the 3 ′ UTR of human APC, 40 ng pGL4.74 hRLuc / TK Renilla luciferase vector (Promega, Fitchburg, WI), and 25 nM hsa - miR - 142 precursor or negative control precursor (Ambion).

Results indicated that 1) the active distraction regulation strategy mediated the relations between early shyness and maternal report of internalizing symptoms; 2) the passive / dependent regulation strategy mediated the relations between shyness and teacher report of internalizing symptoms; and 3) both mediation processes were moderated by maternal negative control.

The number of OTUs present in at least one saliva (red) or maternal (green) or fetal (blue) side fetal sample in both DNA extractions and not in negative controls at that abundance is shown over a range of abundance values (x-axis)

In the qPCR analysis, both the placental samples and contamination controls yielded quite low values, with no clear signal in the placental samples above negative controls.

Paraffin embedded sections, negative control also showing staining, secondary antibody is fine.

Set up negative controls by omitting the primary antibody incubation step for one slide per each experimental condition.

One aspect is describing negative control results, which, in the parlance of a «whodunit,» establishes who didn't do it.

Blood from Zika - infected patients tested positive, but blood from Zika - negative controls did not.

EdiGene Knockout Cell Lysates can be used to validate antibodies for specificity or used as high - quality negative controls for Western blot analysis.

While the trained network accurately reproduced the direction of illusory rotation, it did not detect motion components in negative control pictures wherein people do not perceive illusory motion.

Results were expressed as specific footpad swelling = (24 - hr measurement − 0 - hr measurement) for experimental footpad − (24 - hr measurement − 0 - hr measurement) for negative control footpad.

Each experiment was repeated in triplicate, including a no - template negative control.

We quantified total 16S rRNA gene copies using quantitative PCR and found that placental samples and negative controls contained low and indistinguishable copy numbers.

One current limitation of our study, and of others performing serologic and PCR testing for XMRV, is the absence of bona fide positive and negative control specimens from infected and uninfected humans to determine the analytical sensitivity and specificity of the detection assays.

The human negative control serum was obtained from a healthy volunteer previously determined to be seronegative.

Then, the methylcellulose discs were implanted on the CAM, the eggs were sealed with adhesive tape and returned to the incubator for 48 h. Negative controls were always made with DMSO mixed with the methylcellulose.

The isotype control at 1 ug / mL shows no higher signal than the no primary negative control.

less than 0 The signal from the positive and negative controls overlap, making the assay essentially useless for screening purposes.

All FIV - positive cats in the study receive a veterinary examination every 6 months, including blood and urine collection for hematology, serum biochemistry and urinalysis, while FIV - negative control cats receive this every 12 months.

Parental Negative Control Moderates the Shyness — Emotion Regulation Pathway to School - Age Internalizing Symptoms.

(A) GUS activity with and without 10 µm of the TNT ligand in paired leaves from transgenic plants serving as negative controls and transgenic plants containing the complete signal transduction system.

In addition, a separate set of goat sera was also tested in a blinded fashion and included external positive and negative controls consisting of dilutions of the MuLV whole virus, gp69 / 71 goat polyclonal antisera, or pre-immune goat sera, respectively.

Negative controls including the secondary antibody alone and uninfected monkey tissue were used, as was an ex vivo section of infected macaque brain tissue [43] for the positive control.

To co-transfect pCMV - Neo-APC plasmids, cells were transfected with 200 ng TOPFlash or FOPFlash luciferase expression construct, 20 ng pRL - TK Renilla luciferase vector (Promega), 300 ng pCMV - Neo-APC plasmids, and 25 nM hsa - miR - 142 precursor or negative control precursor (Ambion).

This study tested a moderated mediation model that related early childhood shyness, emotion regulation and maternal negative control to school - age internalizing problems among 257 boys from low - income families.

In summary, all placental and negative control samples were at the extreme low end of the range detectable by qPCR (Additional file 2: Figure S1A — C).

All extractions and amplifications included negative controls at a ratio of one control for every four samples.

All proteins except one (MSRP4) were identified as being immunoreactive as assessed by immunoreactivity > 3 SD above negative control (green bar).

For negative control data where each droplet contains RNA from the same solution, the count depth variability must be technical, but in real samples this could also be due to cells having variable amounts of RNA.