The producer cells express active Cas9 endonuclease which forms a ribonucleoprotein (RNP) complex with your expressed sgRNA. (takarabio.com)
In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end - labeled oligonucleotide probe hybridized in solution to the amplified beta - globin sequences. (science.sciencemag.org)
Burt's notion was to domesticate a microbial family of selfish elements called homing endonuclease genes. (sciencenews.org)