Coupling SRS with alkyne tags has been reported earlier by the same group as a general strategy for imaging small biomolecules. (sciencedaily.com)
Selective hydrogenation is the key to producing valuable chemicals, for example, turning triple - bonded hydrocarbons called alkynes selectively into double - bonded alkenes — starting materials for the synthesis of plastics, fuels and other commercial products. (sciencedaily.com)
Specifically, glucose is labeled with a small alkyne tag (i.e., carbon - carbon triple bond) to generate a strong and characteristic Raman scattering signal in the otherwise silent region, which can be picked up by SRS microscope with high sensitivity and specificity to produce a quantitative concentration map in three dimensions. (sciencedaily.com)