Negative controls consisted of naïve C57BL / 6 mice that had been exposed to BALB / c alloantigens in vivo. (journals.lww.com)
This is in keeping with previous reports indicating that orthotopic corneal allografts in mice do not normally induce detectable CTL responses to donor alloantigens in normal mice and corneal allograft rejection occurs in CD8 KO mice and perforin KO mice, which can not generate allospecific CTL (8, 9). (journals.lww.com)
It was shown that chronically stimulated T cells proliferating in response to alloantigen in the context of GVHD greatly increased both glycolysis and mitochondrial oxidative phosphorylation compared with normal T cells (51). (jimmunol.org)