However, despite multiple serial passages, we were unable to propagate the infected cell culture supernatant in either BSC - 1 or PMK cells. (journals.plos.org)
In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia. (virologyj.biomedcentral.com)
The enveloped viral particles were also observed in negatively stained samples prepared from the concentrated cell culture supernatants and allantoic fluids (Fig. 4F). (journals.plos.org)