Daily medium exchanges help alleviate cellular stresses associated with pH changes and resource consumption due to the high cell density of the culture. (blog.addgene.org)
To keep the cells vital, an optimal cell density of 0.5 — 1.5 × 106 cells per ml medium was maintained. (journals.plos.org)
Both the main strand and the secondary strand, comprising DNA enzymes and the staple sequences, were successfully produced using a high cell density process with bacteria. (sciencedaily.com)