Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits. (sciencedaily.com)
To isolate single colony variants in a non-mutating background, the floxed AID expression cassette was excised by culturing the cells in CM containing 20 nM 4 - hydroxitamoxifen (SIGMA) for three days followed by two rounds of cell sorting for stabilization. (journals.plos.org)
The emission wavelength used for cell sorting of DiI - Ac - LDL - labeled cells was 550 nm. (cancerres.aacrjournals.org)