Cells (5 × 104) were seeded in 12.5 cm2 culture flask and incubated for 24 h to allow for cell attachment and recovery. (wjon.org)
Briefly, the cortices of adult rat (SD) kidneys were collected, minced, and cultured in culture flasks (NUNC, Roskilde, Denmark) using DMEM containing 10 % FBS. (journals.plos.org)
Spleen cells (3 × 107) from either experimental or control effector lymphocytes were dispensed into 75 - cm2 tissue culture flasks along with 20 ml of boosting medium and 6 × 105 γ - irradiated (3,000 rad) BALB / c spleen cells (erythrocytes lysed). (journals.lww.com)