Geniculate ganglia were rapidly excised from CO2 - euthanized mice and placed in enzyme solution [minimum essential medium with Earle's balanced salts (MEM / EBSS; Hyclone) containing 1.25 mg / ml trypsin (Sigma - Aldrich) and 2.5 mg / ml collagenase A (Roche Diagnostics)-RSB- for 30 min. (jneurosci.org)
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Minimum essential medium alpha (MEMα), MEM, Dulbecco's modified Eagle medium / nutrient mixture F12 (DMEM / F12), and fetal bovine serum (FBS) were obtained from Gibco (Grand Island, NY). (oncotarget.com)