The reporter gene construct pXP2 (− 310) was cotransfected with indicated expression plasmids into STAT1 - null U3A cells (C) or IRF - 9 — deficient U2A cells (D). (cancerres.aacrjournals.org)
Even though the work was not hard for me — maintaining mouse strains, isolating DNA, PCR, primer design, preparing to make a new gene construct, northern blots — it took me a lot of time to translate so many English terms into Chinese in my mind. (sciencemag.org)
Prior to transplantation, we analyzed the in vitro production of specific growth factors by unmodified hNPCctx and by hNPCctx transduced with a lentiviral gene construct designed to constitutively express GDNF (hNPCctx - GDNF)[11], [44]. (journals.plos.org)