GFAP (1:5000 mouse monoclonal; Sigma - Aldrich G3893) or CD68 (1:500 rat monoclonal; AbD Serotec MCA1957) were diluted in 3 % normal goat serum with 0.2 % gelatin. (eneuro.org)
Slides were rinsed in PBS three times (5 min each) and blocked in PBS containing 5 % normal horse serum and 1 % normal goat serum. (cancerres.aacrjournals.org)