Recombination between the two target sites with identical sequence leads to inversion of the flanked DNA sequence and the interior target sites. (blog.addgene.org)
The homology - directed repair (HDR) pathway enables the insertion of preplanned genetic modifications using engineered DNA molecules that share identical sequence regions with the targeted gene and which are recognized as a repair template. (sciencedaily.com)
However, given the sequencing depth and the throughput of this instrument, stringent assembly of highly identical sequences can be achieved. (jcvi.org)