The pellet was resuspended in 10 ml of resuspension buffer (20 mM NaHPO4, 300 mM NaCl, 20 mM Imidazole, pH 7.8), and cell lysis was induced by freezing at − 80 °C with PMSF, Leupeptin and Pepstatin added to inhibit protease activity. (journals.plos.org)
This screening tool led to the discovery that a small molecule with a long name, 2 - acetyl4 (5)- tetrahydroxybutyl imidazole, or THI for short, blocks an enzyme that breaks down sphingosine 1 - phosphate. (sciencedaily.com)
Lithographically patterned Pd nanowires were simply overcoated with a Zn - based zeolite imidazole framework (ZIF - 8) layer composed of Zn ions and organic ligands. (sciencedaily.com)