(C and D) Average lesion number (C) and area (D) were quantified using images of immunostaining for RCAS - caErbb2 - HA. (elifesciences.org)
(B - G) Anti-CCHa2 immunostaining of the fat body (B - E) and gut (F, G) from wild - type and CCHa2 mutants. (journals.plos.org)
The morphological identification of eosinophils in H&E stains was supported by using Giemsa stain to highlight intracytoplasmic granules in selected lung sections (not shown), and confirmed by immunostaining with antibodies against mouse eosinophil major basic protein (provided by the Lee Laboratory, Mayo Clinic, Arizona)[36]. (journals.plos.org)