This was done using shRNA technology specific for BRCA1 in human myotubes (skeletal muscle fiber cells). (sciencedaily.com)
A: The GS activities measured at 0.1 mmol / l in the basal state and in the stimulated state in myotubes precultured at the indicated insulin concentration. (diabetes.diabetesjournals.org)
Our dose - response curve at chronic high insulin levels allowed us to differentiate between a primary defect (probably genetic) and the induction of secondary insulin resistance in myotube cultures due to hyperinsulinemia. (diabetes.diabetesjournals.org)