The new and improved ouabain was shown to successfully target sperm - cell proteins in rats, effectively hamstringing their ability to swim, so they couldn't reach and fertilize an egg. (newsweek.com)
Thus, ouabain selection enriches the population for CRISPR edits and improves editing efficiency. (blog.addgene.org)
NHEJ - or HDR - induced mutations in ATP1A1 can render cells resistant to the small molecule ouabain - and editing at the ATP1A1 locus increases the probability that the locus of interest has also been edited! (blog.addgene.org)