The implications of this deduplication requirement, as pointed out by Ng et al, are that the maximum read depth for any given position in the genome is twice the read length for single - end libraries. (massgenomics.org)
Dr. Wullyamoz, of Lausanne, has devised a method of reading this depth directly on a fluorescent screen. (scientificamerican.com)
In the Cohorts arm of the project, we analysed the contribution of low frequency and rare genetic variants to over 50 different cardiometabolic traits using low - read depth whole - genome sequencing. (sanger.ac.uk)