Three oligonucleotides, two primers, and an internal fluorescence - labeled probe for B. burgdorferi (strain N40) ospA and oppA - 2 target genes were designed with Primer Express software (Thermo Fisher Scientific). (journals.plos.org)
In a new study from the laboratory of Gladstone Investigator Katerina Akassoglou, PhD, scientists reveal in animal models that the heightened activity of a protein called thrombin in the brain could serve as an early indicator of MS.. By developing a fluorescently labeled probe specifically designed to track thrombin, the team found that active thrombin could be detected at the earliest phases of MS — and that this active thrombin correlates with disease severity. (gladstone.org)
(c) Nuclear extracts were incubated with the biotin - labeled probes alone (middle lane) or 10-fold molar excess of non-biotin labeled wild - type probes or mutant probes. (nature.com)