To produce the virus, RCASBP plasmids were transfected into DF1 cells, culture medium was harvested, and viral particles were concentrated by ultracentrifugation as described previously (57). (jci.org)
Additionally, the DEF cultures were harvested and concentrated by ultracentrifugation as above. (journals.plos.org)
[3H] TRLs were isolated by buoyant density ultracentrifugation as described above. (jci.org)