Sentences with phrase «+ effector cells»

Syto -59-stained C57BL / 6 CD4 + effector cells were cultured with BALB / c corneal epithelial or endothelial cells overnight.

Not exact matches

The spleen showed increased expansion of antigen - experienced effector and memory CD4 + T - cells.

Single - cell differential gene expression analysis revealed a spectrum of known transcripts, including several linked to cytotoxic and costimulatory function that are expressed at higher levels in the TEMRA (effector memory T cells expressing CD45RA) subset, which is highly enriched for CD4 - CTLs, compared with CD4 + T cells in the central memory (TCM) and effector memory (TEM) subsets.

Calmodulin (CaM) is a major effector for the intracellular actions of Ca2 + in nearly all cell types.

The phenotype of NP cells generated ex vivo (Figure S8) closely resembles that of central memory CD4 + T cells found in vivo, which persist for years in secondary lymphoid organs and can differentiate into effector memory CD4 + T cells [45].

The development of GVHD was equivalent between the two groups (data not shown), suggesting that treatment with X4 - ZFNs did not affect CD4 + T cell effector functionality.

Elite controllers with low to absent effector CD8 + T cell responses maintain highly functional, broadly directed central memory responses.

Rejection by CD8 + T cells does not appear to involve either allospecific CTL or DTH effector mechanisms; however, CD8 + T cells are able to induce significant apoptosis of allogeneic corneal endothelial cells.

After encountering HO - 1 (+) MoDCs, effector T - cells exhibited a lower velocity and a reduced ability to migrate towards chemokine gradients resulting in impaired accumulation to the inflamed organ.

Whereas acute CD8 + T cell effector responses are enhanced in DGKζ - deficient mice postinfection with intracellular pathogens, persistent memory formation is impaired, most dramatically when both DGKζ and DGKα, the other isoform of DGK that metabolizes DAG downstream of the TCR in T cells, are deleted (35, 36).

Graphs show the frequency of IFN - γ effector CD8 + T cells.

Although similar numbers of total splenic gp33 - LCMV — specific T cells were observed between genotypes (Fig. 5B), the distribution of gp33 - LCMV — specific CD8 + T cell effector and memory subsets were altered, such that there was a significantly lower percentage of short - term effector T cells and reciprocal changes in memory precursor cells among gp33 - specifc CD8 + T cells in WT relative to DKO mice (Fig. 5C), with a trend in changes in absolute cell numbers, consistent with temporal data from peripheral blood (Fig. 5A).

Six weeks postinfection, splenocytes were analyzed for gp33 - LCMV — specific total CD8 + T cells (B) or short - lived effector cells and memory precursor effector cells (C).

Consistent with prior reports on effector cells (35), we observed that, among CD8 + T cells specific for the dominant epitope of LCMV (GP33), the percentages of short - lived effector cells (KLRG1 + CD127 −) were increased, and the percentages of memory precursor cells (KLRG1 + CD127 +) were decreased in the peripheral blood of DGKζ − / −, Cbl - b − / −, and DKO mice relative to WT mice after acute infection (Fig. 5A).

Glucose deprivation inhibits multiple key gene expression events and effector functions in CD8 + T cells.

Unexpected positive control of NFκB and miR - 155 by DGKα and ζ ensures effector and memory CD8 + T cell differentiation.

DKO CD8 + T cells demonstrate a strong effector response but altered memory differentiation and maintenance after LCMV infection.

EOS100850 potently rescues Th1 cytokine production in human whole blood treated by A2A agonists, and increased CD8 + T cell cytotoxicity in a co-culture assay of effector CD8 + T cells and target cancer cells

Because treatment with rapamycin also led to decreased AKT phosphorylation in ALK + ALCL cells in our in vitro study, it is tempting to speculate that an effector protein downstream of mTOR - raptor may contribute directly or indirectly to AKT activation.

Thus, VPA did not reduce GVHD by inducing apoptosis of CD4 + effector T cells.

Because of their ability to differentiate into various effector T subsets, Th1, Th2, Th17, and Tregs, CD4 + T cells are particularly important in the initiation of GVHD in mice and have been considered potential targets for the treatment and prevention of GVHD in the clinic.

Delayed differentiation of potent effector CD8 + T cells reducing viremia and reservoir seeding in acute HIV infection.

Effector memory CD4 + T cells mediate graft - versus - leukemia without inducing graft - versus - host disease.

Furthermore, CMV - specific CTL have a highly polarized membrane phenotype that is typical of effector memory cells (CD28 -LRB--), CD57 (+), CCR7 -LRB--)-RRB-.

We explored how aging affects transcriptional dynamics using single - cell RNA sequencing of unstimulated and stimulated naïve and effector memory CD4 (+) T cells from young and old mice from two divergent species.