Sentences with phrase «+ mouse lung»
Nichane et al.: Isolation and 3D expansion of multipotent Sox9 + mouse lung progenitors.
https://www.jax.org/ Not exact matches
This experiment uses quantitative PCR to detect the expression level of POSTN in CD34 + / CD31 − pulmonary fibroblasts, CD31 + endothelial cells, and CD45 + immune cells isolated from lungs of mice with macrometastases, which is a replication of the experiment reported in Figure 2H.
Immediately after counting macrometastasis, use the first six lungs identified from MMTV - PyMT + / tg; Postn + / + female mice that are positive with metastatic disease for further analysis (Protocol 2).
Using the MMTV - PyMT mouse breast cancer model, which spontaneously metastasizes to the lungs, Malanchi and colleagues reported that only the CSC population, identified as CD24 + CD90 +, were capable of initiating lung metastases and secondary metastases (Guy et al., 1992; Lin et al., 2003; Malanchi et al., 2012).
We found no change in the total number of ILCs (Lin - CD45.2 + Thy1.2 + CD127 +) in the spleens, lungs or livers of toxin treated mice compared to mice that were injected with vehicle control (Fig 6A and S6A Fig).
(B) Representative images of the excised tumours and lung metastatic nodules (black arrows) from 4 - month - old Slc6a14 + / + / PyMT mice and Slc6a14 − / − / PyMT mice.
In 4 - month - old PyMT - Slc6a14 + / + mice, metastatic nodules were detectable in the lung but there was no metastasis in age - matched PyMT - Slc6a14 − / − mice (Figure 2B).
In the November 6 Nature Methods, the team led by Bing Lim and Massimo Nichane of the Genome Institute of Singapore and Kyle M. Loh of Stanford University School of Medicine, and including Siva V, Ph.D., of The Jackson Laboratory's Single Cell Biology Lab, showed that mouse Sox9 + multipotent embryonic lung progenitors can be isolated and expanded long - term in 3D culture.