In vivo fractional synthesis rates (FSR, % / d) were calculated according to the method described by Garlick et al. (11): FSR
= 100 × (EP − EN) / (EA × t), where t is the incorporation time (15 min), expressed in days, EP is the enrichment of protein - bound
valine at the time of killing, EN is the natural enrichment of protein - bound
valine, which was measured in each tissue in 5 rats fed the same fish diet and EA is the enrichment of tissue - free
valine at the time of killing.