Antigen retrival was with
10mM citrate pH6.0 (heat mediated).
Boil tissue section in
10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Not exact matches
Tissue was fixed with 10 % buffered formalin and blocked with 5 % serum for 60 minutes at 21 °C; antigen retrieval was by heat mediation in a
10mM sodium
citrate buffer pH6.