(REgularised LIkelihood OptimisatioN) a stand - alone computer program for Maximum A Posteriori (MAP) refinement of (multiple) 3D reconstructions or 2D class averages in cryo - electron microscopy.
(Left) Representative reference - free 2D class averages of negative - stain electron microscopy (EM) images of the uncleaved BG505 gp140 and the native - like BG505 SOSIP.664 trimers.