Another popular technique for
3D cell imaging called spinning disc confocal microscopy can't image samples nearly as long as Betzig's technique, but it is still better for imaging thick cells and tissue.
Not exact matches
Companies making high - content
cell imaging equipment such as confocal microscopes and plate readers are now adding software tools to process images of
3D cell cultures.
Using this, the researchers performed
3D super-resolution
imaging of stained structures in the
cells, and combined it with
3D label - free phase
imaging.
They examined the pattern of slime thread coiling within developing
cells using light and electron microscopy and
3D imaging and modelling.
The speed, noninvasiveness, and high spatial resolution of this approach make it a promising tool for in vivo
3D imaging of fast dynamic processes in
cells and embryos, as shown here in five surrounding examples.
The combination of two processes makes this high - resolution
3D imaging possible: lattice light - sheet microscopy (LLSM), which images one slice of the
cell at a time, and adaptive optics (AO), which corrects for any blurriness.
diSPIM technology enables rapid
3D imaging of samples ranging from single
cells to small organisms over the course of hours to days.
Initial
imaging analysis of fluorescently labelled human iPSC - derived hepatic endoderm
cells, umbilical cord - derived endothelial
cells (HUVECs), and mesenchymal stem
cells (MSCs) co-cultured in a solidified matrix gel to promote
3D growth found that the different
cells collectively and automatically «condensed» into a multicellular central unit.
This article describes methods for preparation and
imaging of
3D cancer
cell cultures, either as single
cells or spheroids.
Our results demonstrated that our
3D cell culture device enables a real - time
imaging of giant vacuole formation and tracers crossing the cultured endothelial
cell monolayer in a controlled experimental condition.
By combining two state - of - the - art
imaging technologies, Howard Hughes Medical Institute Janelia Research Campus scientists, led by 2014 chemistry Nobel laureate physicist Eric Betzig, have imaged living
cells at unprecedented
3D detail and speed, the scientists report on April 19, 2018 in an open - access paper in the journal Science.
Additional confocal applications include microirradiation to study DNA - damage repair,
3D reconstructions and time - lapse
imaging of dynamic
cell and molecular processes.
Read more about Real - time
3D imaging of microstructure growth in battery
cells using indirect MRI