To highlight the potentially important role of
3D culture conditions, the authors compared 2D monolayers of hepatocyte progenitors, fetal liver stromal cells, or adult bone marrow - derived stromal progenitor cells to their 3D counterparts cultured on liver extracellular matrix (3D - ECM) constructs.
Our results demonstrated that our
3D cell
culture device enables a real - time imaging of giant vacuole formation and tracers crossing the
cultured endothelial cell monolayer in a controlled experimental
condition.