Sentences with phrase «a3 wt»
The ability of the ospC7 / ospC +4 clone to infect mice was tested by needle - inoculation with a moderate i.p. / s.c. dose of 5 × 103 ospC7 / ospC +4 organisms, in parallel with the isogenic B31 - A3 WT and ospC7 mutant clones (Table 1).
http://www.pnas.org/
Consistent with this theoretical consideration, analysis of gene products from adjacent loci (guaA / B and BBB22) demonstrated comparable levels in B31 - A3 WT, ospC mutant, and complemented bacteria (data not shown).
Mice were inoculated with 5 × 103 B31 - A3 WT or ospC mutant bacteria by i.p. / s.c. injection.
The ospC mutant and B31 - A3 WT had comparable in vitro doubling times.
(A) IFA of midgut tissues from partially fed nymphal ticks infected with B31 - A3 WT, ospC7 mutant, or ospC7 / ospC +4 complemented B. burgdorferi clones.
Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.