Sentences with phrase «aav1 supernatant»

«It's an interesting project because backwash supernatant disinfection is not widely used in Australia,» said Michael Bambridge, Managing Director of CST Wastewater Solutions.

A major NSW regional council has introduced a supernatant recycling system into the filtration plant in the town water supply, helping to conserve potable water and providing substantial water savings over the long term.

Hybridoma supernatants were screened for anti-rGRF activity by use of a pituitary culture assay system that can detect growth hormone - releasing factor in the femtomole range.

By securing a pellet (with neodymium magnets) on the sides of the tube walls rather than at the bottom, it allows complete removal of the supernatant without touching the pellet.

With the current designs of magnetic stands, these purifications can be problematic, often leaving carryover contaminants when the pellet is disturbed or when the supernatant is not completely removed.

After achieving 80 — 90 % confluency, cell culture media were changed to maintenance media with 2 % FBS and were inoculated with 200 µL of clinical sample or 100 µL of passaged viral supernatant.

To confirm the generation of infectious virus, viral supernatants were quantitated by an end - point dilution assay.

The supernatants were discarded, and the tissue was resuspended in DMEM supplemented with 10 % fetal bovine serum, 2 - mm l - glutamine, sodium pyruvate, nonessential amino acids, and a vitamin solution (Life Technologies, Inc., Rockville, MD).

For each pool, 55 µL of rabbit sera and 55 µL of viral supernatant at a concentration of 100 TCID50 were mixed, incubated for 1 hour at 37 °C, and inoculated onto A549 cells in wells of a 96 - well plate as described above.

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

To perform the virus neutralization assay, 55 µL of viral supernatant at a concentration of 100 TCID50 and 55 µL of serum (starting at a 1 ∶ 8 dilution) were mixed and incubated for 1 hour at 37 °C.

Mcc was purified from the culture supernatants of E. coli VCS257 cells harboring pJEM15 plasmid, as described earlier (Bieler et al., 2005).

Both systems can simultaneously detect many targets such as cytokines, chemokines and inflammatory biomarkers in a single sample that can be serum, plasma and tissue culture supernatants.

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

The group has over 10 years» experience with this methodology, with specific expertise in cytokine / chemokine detection in tissue culture supernatants and plasma samples with panels containing up to 42 analytes.

After the third wash, the supernatant was discarded down to 100 µl and 650 µl of Tris - HCL buffer was added followed by 250 µl of fresh / frozen reduction buffer (100 mM Tris - Cl pH 7.4, 40 mM DTT).

Supernatants were us

Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet.

Supplied as cell free supernatant from H9's or infected H9 cells.

(E and F) Using ELISA, IL - 2 and IFN - γ production were measured from culture supernatants of (D) after 72 h of stimulation.

Both technologies allow the simultaneous detection of multiple factors in serum, plasma and tissue culture supernatants.

This supernatant was thawed on ice and 50 µl added to 400 µl HPLC grade water, 50 µl of 50 % MPA and 200 µl of HPLC grade heptane.

Maternal plasma previously acidified 1 ∶ 1 with ice - cold 10 % metaphosphoric acid (MPA) was centrifuged and the supernatant stored at − 70 °C.

After a 1 min centrifugation (10,000 g) step, the supernatant was transferred into a fresh tube and placed on ice.

Phosphorylation of supernatant protein factor enhances wits ability to stimulate microsomal squalene monooxygenase.

Cultured pericytes abundantly expressed calpain1 (Fig. 2d) and showed a significant induction of calpain1 activity after stimulation with supernatants of Ang2 overexpressing HUVEC (Fig. 2e).

Vaccinia virus (Western Reserve) recombinant for the codon - optimised gp120 gene from HIV - 1 (Bx08) expressing gp120 protein in (VERO) cell culture supernatant.

IFN - γ and IL - 2 production from supernatants of individual wells was determined using an ELISA (BioLegend, San Diego, CA).

Loaded gesicles can then be collected from the supernatant and added to target cells for efficient editing with no footprint.

Our lab uses this kit routinely to to quantify p24 levels in culture supernatant.

Freeze supernatant 10 days into the co-culture.

Furthermore, an increased amount of IL - 2 was present in the supernatants of anti-CD3 — stimulated DKO and Cbl - b − / − — stimulated CD8 + T cells relative to DGKζ − / − CD8 + T cells, which was increased relative to WT CD8 + T cells (Fig. 3E).

Angiopoietin - 2 protein levels of cell culture supernatants were measured by ELISA (R&D, #DANG20) according to the manufacturer's instructions.

I can confirm that the Anti-CD3 antibody [SP7](ab16669) antibody isprovided as tissue culture supernatant.

The supernatant of apoptotic cells causes transcriptional activation of hypoxia - inducible factor — 1α in macrophages via sphingosine -1-phosphate and transforming growth factor - β.

Stimulation of pericytes with supernatants from either Ang2 overexpressing HUVEC or supernatants from control HUVEC resulted in significantly increased pericyte migration following Ang2 stimulation (Fig. 2c, Supplementary Fig. 4c, d).

Supernatants were collected 24 and 48 h after transfection.

Gesicles can be collected from the supernatant, yielding a concentrated stock of your Cas9 - sgRNA gesicles.

One example is SPF, or supernatant protein factor, which transfers substrate from an inactive to an active pool or from one enzyme site to another.

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.

Briefly, the appropriate dilution of tissue supernatant was added to the cuvette containing 1.9 mL of 50 mmol phosphate buffer (pH 7.0).

Homogenates were cleared by centrifugation at 100.000 g for 1 h at 4 °C, and supernatants were collected for further analysis (TBS - soluble fraction).

Briefly, 100 ml of culture supernatants were mixed with AVL - Carrier RNA buffer.

RNA was also extracted from supernatant of mosquitoes pools as described above and used for the ZIKV rRT - PCR assay.

Centrifugation at 100.000 g was repeated and supernatants were collected (FA - soluble fraction).

To remove hepatocytes, homogenates were centrifuged at 300 rpm for 3 min, and then supernatants were centrifuged at 1500 rpm for 10 min.

Spleen CD4 + T cells isolated from BMT recipients on day 21 were treated with vehicle solution or VPA for 24 h, the supernatants were collected, and ELISA was performed to determine the level of IFN - γ (C) and IL - 17A (D).

Five hours later, the supernatants were collected to determine cytokine levels by ProcartaPlex Multiplex Immunoassays.

For the total input control, 20 % of the total supernatant was saved and frozen at − 80 °C.

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.