Sentences with phrase «ack lysis buffer»
Spleens were then treated with ACK lysis buffer to remove RBCs.
http://www.immunohorizons.org/
Spleens were processed into cell suspensions through a 70 - μm strainer by a syringe plunger in RPMI 1640 with 10 % FBS and then treated with ACK lysis buffer to remove RBCs.
Peripheral blood mononuclear cells were isolated by centrifugation with 96 % Ficoll (BD), followed by erythrocyte lysis with ACK lysis buffer.