Sentences with phrase «adep efflux pump»

Fortunately, efflux pumps require a source of chemical energy to perform their function.

To address this challenge, our research group is using nanoparticles not only to deliver more chemotherapy drugs to the target site within cancer cells, but also to compromise the function of the efflux pumps and thereby significantly improve safety and efficacy of cancer therapy.»

Recognizing this, He and his research team developed a way to reduce the amount of chemical energy — adenosine triphosphate (ATP)-- available to the efflux pumps in cancer cells.

The AcrA - PPMO also was effective against the human pathogens Klebsiella pneumoniae and Salmonella enterica, since those bacteria contain the same efflux pump with a matching gene sequence, Dr. Greenberg said.

The UTSW experiment focused on Escherichia coli (E. coli) and bacterial pathogens with genetically similar efflux pump complexes.

Bacteria that use these «efflux pumps» and that are treated with the new compound become sensitive to antibiotics they were previously resistant to.

Studies have revealed that the drug also inhibits bacterial efflux pumps that permit bacteria to survive within cells.

The findings, published November 28 in Scientific Reports, offer a better understanding of the motions and functions of efflux pumps.

If a pathogenic strain of bacteria acquires a gene encoding one of these efflux pumps, then it will suddenly become resistant to multiple antibacterial drugs.

«There are two scenarios for how an ADEP efflux pump could operate,» Sello said.

The efflux pumps are kept busy pumping out the fragments while the antimicrobial agents are able to stay inside the cell.

One type of efflux pump, which until recently had only been studied in parts, was recently modeled in its entirety and simulated using supercomputers at Los Alamos National Laboratory.

Application of these computational techniques to the multitude of efflux pumps found in different Gram - negative pathogens should allow scientists to elucidate if general mechanisms are shared among different pumps or are pump - specific.

«There are efflux pumps that can export multiple drugs that have different structures and mechanisms of action.

Bacterial efflux pumps, such as the P. aeruginosa MexAB - OprM pump shown here, are one of the dominant molecular mechanisms available to Gram - negative pathogens for removing toxins, including antibiotics.

For this study, the researchers focused on efflux pumps inside the bacteria Pseudomonas aeruginosa, which can cause serious illnesses such as pneumonia and sepsis.

«We study a lot of efflux pumps to understand antibiotic resistance,» said Yu, an Iowa State professor with appointments in physics and astronomy; chemistry; biochemistry, biophysics and molecular biology; and the U.S. Department of Energy's Ames Laboratory.

New supercomputer simulations have revealed the role of transport proteins called efflux pumps in creating drug - resistance in bacteria, research that could lead to improving the drugs» effectiveness against life - threatening diseases and restoring the efficacy of defunct antibiotics.

It had been reported that the ADEP resistance of M. tuberculosis was due to the presence of one or perhaps more efflux pumps.

Since an efflux pump could compromise the potency of even a newly developed antibacterial drug, we need to think about ways to circumvent these efflux pumps.»

One trick used by some bacteria is to eject antibiotics through so - called efflux pumps embedded in their cellular membranes.

Some coded for multi-drug efflux pumps, which are multi-drug resistance mechanisms embedded in the bacterial membrane that pump specific antibiotics out of the bacteria.

Another supplied a plant compound (diosmetin) known to disable efflux pumps in other bacteria.

The Solution: Working with pathogenic bacteria would be far too dangerous in a school setting, so Delgado focused on disabling an efflux pump in the common bacterium E. coli.

Heterogeneity in a bacterial population that arises through a mechanism of biased partitioning of drug efflux pumps, as we identified in our study, could be a stepping - stone on the path of bacterial populations towards antibiotic resistance.»

Saint Louis University researchers then synthesized structural analogs of the discovered efflux pump inhibitors and identified properties essential for their activities.

The team focused on one efflux pump protein, known as AcrA, which connects two other proteins in a tunnel shape through the bacterial cell envelope.

«After more extensive analysis, we narrowed down our list to predict which molecules were most likely to disrupt the function of the efflux pump.»

«As a first in this field, we proposed the approach of essentially «screwing up» the efflux pump's protein assembly, and this led to the discovery of molecules with a new type of antibacterial activity,» said co-author Jeremy Smith, who serves as a UT - ORNL Governor's Chair and director of the UT - ORNL Center for Molecular Biophysics.

The researchers found four new chemicals that seek out and disrupt bacterial proteins called «efflux pumps,» known to be a major cause of antibiotic resistance.

They plan to leverage a recently awarded Innovative and Novel Computational Impact on Theory and Experiment (INCITE) allocation from DOE to perform larger simulations on the Titan supercomputer to gain deeper understanding of how bacterial efflux pumps function, identify more potent efflux pump inhibitors and optimize the best antibiotic - plus - inhibitor combinations to make them suitable for clinical trials.

This picture was suddenly complicated in 1999, when Gerd Jürgens and his colleagues at the University of Tübingen in Germany discovered a mutant of the model plant Arabidopsis with efflux pumps everywhere in the cell.

The TB bacteria use efflux pumps as a strategy to remove drugs and other threats from the cell before they can work against the bacteria.

Similarly in C. neoformans, Chr 1 disomy confers azole resistance because this chromosome harbors ERG11 and AFR1 (which encodes the major azole efflux pump) and aneuploidy can also influence the virulence of this pathogen [16], [20], [21].

Efflux pumps are surface proteins that prevent antimicrobial drugs from getting a foothold in a bacterial cell by identifying and pumping them out of the cell.

Among other things, we have identified one mechanism of multi-drug resistance (MDR) involving P - glycoprotein (Pgp) which functions as a drug efflux pump of broad specificity.

Furthermore, if an agent is able to penetrate one or both of these membranes, it is often subject to rapid efflux out of the cell by numerous broadly acting efflux pumps - rendering the agent ineffective.

Additionally, cells treated with MIA - 602 showed a significant increases in Calcein dye retention, indicating that efflux pump function was reduced.

PP: Animal studies, efflux pump assay, and manuscript preparation.

The genes MDR1 and NANOG, both involved in the regulation and expression of efflux pump mechanisms, were significantly suppressed.

: This study evaluated the effects of an antagonistic analog of growth hormone - releasing hormone, MIA - 602, on tumor growth, response to doxorubicin, expression of drug resistance genes, and efflux pump function in human triple negative breast cancers.

Introduction: This study evaluated the effects of an antagonistic analog of growth hormone - releasing hormone, MIA - 602, on tumor growth, response to doxorubicin, expression of drug resistance genes, and efflux pump function in human triple negative breast cancers.

We examined the effects of treatment on tumor growth, drug resistance, GHRH - R levels, expression of MDR1 and Nanog, and efflux pump activity.

In - vitro cell culture assays were used to analyze the effect of MIA - 602 on efflux pump function.

MIA - 602 also suppressed efflux pump function in both cell lines.

For those that are not having significant changes by 3 months then I am adding Biocidin to remove biofilms and to decrease antibiotic resistance by blocking efflux pumps that remove antibiotics from germs.

decreases drug resistance in Lyme by blocking efflux pumps that remove antibiotics from germs,

Based on unpublished lab experiments Biocidin breaks down biofilms and decreases antibiotic resistance by blocking efflux pumps that remove antibiotics from germs.

Germs in biofilms use efflux pumps that flush antibiotics out.

That's kind of what efflux pumps do.