Sentences with phrase «alk +»

However, the activation status of the mTOR pathway in ALK + ALCL is unknown.

Of note, AKT activation in ALK + ALCL tumors, determined as Ser473p - AKT expression (20), significantly correlated with p - mTOR and p - rpS6 (P = 0.037 and 0.002, respectively, χ2 test) as well as with total 4E - BP1 and total eIF4E (P = 0.001 and 0.003, respectively, χ2 test).

C and D, the phosphorylation status of mTOR, p70S6K, 4E - BP1, and rpS6 was assessed immunohistochemically in a series of 31 ALK + ALCL tumors.

In ALK + ALCL, the chimeric protein nucleophosmin - ALK has been shown to activate PI3K and its downstream effector AKT (4).

A and B, two ALK + ALCL cell lines, Karpas 299 and SU - DHL1, were treated with the selective PI3K inhibitor, LY294002, at a concentration of 0, 5, 10, or 20 μg / μL.

p - 4E - BP1 and total eIF4E were both positive in all 31 ALK + ALCL tumors examined, but the level of detection was variable.

mTOR pathway is frequently activated in ALK + ALCL tumors and cell lines.

However, the activation status of the mTOR pathway has not been investigated in ALK + ALCL.

We also show that inhibition of mTOR with rapamycin, as well as silencing mTOR gene product expression using mTOR - specific small interfering RNA, decreased phosphorylation of mTOR signaling proteins and induced cell cycle arrest and apoptosis in ALK + ALCL cells.

Inhibition of mTOR induces apoptosis in ALK + ALCL cells.

We observed that the phosphorylation level of AKT significantly correlated with the phosphorylation level of mTOR and rpS6 as well as with expression of total 4E - BP1 and eIF4E in ALK + ALCL tumors.

The phosphorylation status of mTOR, 4E - BP1, p70S6K, and rpS6 was immunohistochemically examined in 31 ALK + ALCL tumors.

Immunohistochemical methods were used to assess protein expression in formalin - fixed, paraffin - embedded cell blocks from Karpas 299 and SU - DHL1 and in 31 ALK + ALCL tumors using methods described previously (21).

Inhibition of the mTOR pathway interferes with essential proliferation and survival pathways in ALK + ALCL and might form the basis for a novel therapeutic strategy.

To investigate if AKT activation is sufficient to activate the mTOR pathway in ALK + ALCL cells, we infected the Karpas 299 and SU - DHL1 cell lines with an adenoviral vector, adeno - myrAkt, expressing constitutively active AKT (23).

These results suggest that inhibition of mTOR - raptor complex or silencing mTOR gene expression induced cell cycle arrest at G1 phase in ALK + ALCL cells.

We also provide evidence that activation of the mTOR pathway in ALK + ALCL depends, at least in part, on PI3K / AKT.

mTOR signaling in ALK + ALCL oncogenesis.

We also show that rapamycin and total mTOR silencing induces cell cycle arrest and apoptosis interfering with proliferation and survival pathways in ALK + ALCL.

Our results confirm that rapamycin inhibits activation of the mTOR pathway in ALK + ALCL cells.

Two ALK + ALCL cell lines were used, Karpas 299 and SU - DHL1.

These findings suggest that activation of mTOR signaling contributes to tumor cell survival in ALK + ALCL, thus providing a potential therapeutic target in this lymphoma type.

Because treatment with rapamycin also led to decreased AKT phosphorylation in ALK + ALCL cells in our in vitro study, it is tempting to speculate that an effector protein downstream of mTOR - raptor may contribute directly or indirectly to AKT activation.

In this study, we hypothesized that the mammalian target of rapamycin (mTOR) pathway, which functions downstream of AKT, mediates the oncogenic effects of activated PI3K / AKT in ALK + ALCL.

Here, we show that the mTOR pathway is activated in ALK + ALCL.

Here, we provide evidence that mTOR signaling phosphoproteins, including mTOR, eukaryotic initiation factor 4E — binding protein - 1, p70S6K, and ribosomal protein S6, are highly phosphorylated in ALK + ALCL cell lines and tumors.

Toyokawa G, Seto T, Takenoyama M, Ichinose Y. Insights into brain metastasis in patients with ALK + lung cancer: is the brain truly a sanctuary?

Phase I / II clinical trial results reported at the American Society for Clinical Oncology (ASCO) Annual Meeting 2015 show promising results for investigational drug brigatinib against ALK + non-small cell lung cancer (NSCLC), with 58 of 78 ALK + patients responding to treatment, including 50 of 70 patients who had progressed after previous treatment with crizotinib, the first licensed ALK inhibitor.

Not exact matches

Abnormalities that are commonly seen in Cushing's patients are: Elevated liver enzymes (Alk Phos, + / - ALT), + / - increased blood glucose, unconcentrated urine (specific gravity less that 1.025), protein in urine, Alterations in blood cell counts (increased neutrophils, decreased lymphocytes and eosinophils), and + / - nucleated red blood cells.

ACT - activated clotting time (bleeding disorders) ACTH - adrenocorticotropic hormone (adrenal gland function) Ag - antigen test for proteins specific to a disease causing organism or virus Alb - albumin (liver, kidney and intestinal disorders) Alk - Phos, ALP alkaline phosphatase (liver and adrenal disorders) Allergy Testing intradermal or blood antibody test for allergen hypersensitivity ALT - alanine aminotransferase (liver disorder) Amyl - amylase enzyme — non specific (pancreatitis) ANA - antinuclear antibody (systemic lupus erythematosus) Anaplasmosis Anaplasma spp. (tick - borne rickettsial disease) APTT - activated partial thromboplastin time (blood clotting ability) AST - aspartate aminotransferase (muscle and liver disorders) Band band cell — type of white blood cell Baso basophil — type of white blood cell Bile Acids digestive acids produced in the liver and stored in the gall bladder (liver function) Bili bilirubin (bile pigment responsible for jaundice from liver disease or RBC destruction) BP - blood pressure measurement BUN - blood urea nitrogen (kidney and liver function) Bx biopsy C & S aerobic / anaerobic bacterial culture and antibiotic sensitivity test (infection, drug selection) Ca +2 calcium ion — unbound calcium (parathyroid gland function) CBC - complete blood count (all circulating cells) Chol cholesterol (liver, thyroid disorders) CK, CPK creatine [phospho] kinase (muscle disease, heart disease) Cl - chloride ion — unbound chloride (hydration, blood pH) CO2 - carbon dioxide (blood pH) Contrast Radiograph x-ray image using injected radiopaque contrast media Cortisol hormone produced by the adrenal glands (adrenal gland function) Coomb's anti- red blood cell antibody test (immune - mediated hemolytic anemia) Crea creatinine (kidney function) CRT - capillary refill time (blood pressure, tissue perfusion) DTM - dermatophyte test medium (ringworm — dermatophytosis) EEG - electroencephalogram (brain function, epilepsy) Ehrlichia Ehrlichia spp. (tick - borne rickettsial disease) EKG, ECG - electrok [c] ardiogram (electrical heart activity, heart arryhthmia) Eos eosinophil — type of white blood cell Fecal, flotation, direct intestinal parasite exam FeLV Feline Leukemia Virus test FIA Feline Infectious Anemia: aka Feline Hemotrophic Mycoplasma, Haemobartonella felis test FIV Feline Immunodeficiency Virus test Fluorescein Stain fluorescein stain uptake of cornea (corneal ulceration) fT4, fT4ed, freeT4ed thyroxine hormone unbound by protein measured by equilibrium dialysis (thyroid function) GGT gamma - glutamyltranferase (liver disorders) Glob globulin (liver, immune system) Glu blood or urine glucose (diabetes mellitus) Gran granulocytes — subgroup of white blood cells Hb, Hgb hemoglobin — iron rich protein bound to red blood cells that carries oxygen (anemia, red cell mass) HCO3 - bicarbonate ion (blood pH) HCT, PCV, MHCT hematocrit, packed - cell volume, microhematocrit (hemoconcentration, dehydration, anemia) K + potassium ion — unbound potassium (kidney disorders, adrenal gland disorders) Lipa lipase enzyme — non specific (pancreatitis) LYME Borrelia spp. (tick - borne rickettsial disease) Lymph lymphocyte — type of white blood cell MCHC mean corpuscular hemoglobin concentration (anemia, iron deficiency) MCV mean corpuscular volume — average red cell size (anemia, iron deficiency) Mg +2 magnesium ion — unbound magnesium (diabetes, parathyroid function, malnutrition) MHCT, HCT, PCV microhematocrit, hematocrit, packed - cell volume (hemoconcentration, dehydration, anemia) MIC minimum inhibitory concentration — part of the C&S that determines antimicrobial selection Mono monocyte — type of white blood cell MRI magnetic resonance imaging (advanced tissue imaging) Na + sodium ion — unbound sodium (dehydration, adrenal gland disease) nRBC nucleated red blood cell — immature red blood cell (bone marrow damage, lead toxicity) PCV, HCT, MHCT packed - cell volume, hematocrit, microhematocrit (hemoconcentration, dehydration, anemia) PE physical examination pH urine pH (urinary tract infection, urolithiasis) Phos phosphorus (kidney disorders, ketoacidosis, parathyroid function) PLI pancreatic lipase immunoreactivity (pancreatitis) PLT platelet — cells involved in clotting (bleeding disorders) PT prothrombin time (bleeding disorders) PTH parathyroid hormone, parathormone (parathyroid function) Radiograph x-ray image RBC red blood cell count (anemia) REL Rocky Mountain Spotted Fever / Ehrlichia / Lyme combination test Retic reticulocyte — immature red blood cell (regenerative vs. non-regenerative anemia) RMSF Rocky Mountain Spotted Fever SAP serum alkaline phosphatase (liver disorders) Schirmer Tear Test tear production test (keratoconjunctivitis sicca — dry eye,) Seg segmented neutrophil — type of white blood cell USG Urine specific gravity (urine concentration, kidney function) spec cPL specific canine pancreatic lipase (pancreatitis)-- replaces the PLI test spec fPL specific feline pancreatic lipase (pancreatitis)-- replaces the PLI test T4 thyroxine hormone — total (thyroid gland function) TLI trypsin - like immunoreactivity (exocrine pancreatic insufficiency) TP total protein (hydration, liver disorders) TPR temperature / pulse / respirations (physical exam vital signs) Trig triglycerides (fat metabolism, liver disorders) TSH thyroid stimulating hormone (thyroid gland function) UA urinalysis (kidney function, urinary tract infection, diabetes) Urine Cortisol - Crea Ratio urine cortisol - creatine ratio (screening test for adrenal gland disease) Urine Protein - Crea Ratio urine protein - creatinine ratio (kidney disorders) VWF VonWillebrands factor (bleeding disorder) WBC white blood cell count (infection, inflammation, bone marrow suppression)