Cysteine - to -
Alanine substitutions (C435A, C461A, C492A and C646A) in the CP motifs were achieved through site - directed mutagenesis using the QuikChange II Site Directed Mutagenesis Kit from Agilent Technologies.
To probe the role of these CP motifs in modulation of Bach1 by HPP - 4382, a mutant Bach1 protein containing Cysteine to
Alanine substitutions at CP motifs 4 through 7 was constructed (Figure 6A).
Not exact matches
They are of high interest for the study of complex diseases that have no clear mutational cause,» said Yamashita, who added the
alanine threonine
substitution was a rare variant.