Sentences with phrase «amoebocyte lysate»

Using the kits, you can quickly and easily label proteins in lysates or purified proteins with near - infrared CF ® dyes before electrophoresis and blotting.

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

A transmembrane form is the predominant product generated in wheat germ (WG) extracts, whereas a completely translocated (secretory) form is the major product synthesized in rabbit reticulocyte lysates (RRL).

But after a while, I realized that I understood the rabbit reticulocyte lysate system much better than anybody else in the entire world, because I had made every single mistake that was possible to make and learned from them.

They typically show up on ingredient labels as «plankton extract» or «micrococcus lysate.»

EdiGene Knockout Cell Lysates can be used to validate antibodies for specificity or used as high - quality negative controls for Western blot analysis.

Each lysate product is sold in kit format consisting of a KO cell lysate and a parental cell lysate, which are immediately available from OriGene for the academic and industrial research markets.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

The research, performed using recombinant protein from cell lysate isolated in vitro, also validates a methodology for characterizing large, multivalent membrane proteins in general.

Target detectability data from 858 samples from 11 independent customer sites, utilizing five unique sample types: serum, plasma, cell culture supernatant, lysates, and bronchoalveolar lavage.

The lysates were then incubated on ice at 4 °C for 30 min and centrifuged for 5 min at 500x g at 4 °C.

Samples matrixes suitable for metabolomics analyses include in vitro: cell lysate and medium, and in vivo: Plasma, serum, urine, CSF, micro-dialysate and tissues

William B. Coley creates a filtered mixture of bacteria and bacterial lysates, composed of Streptococcus pyogenes and Bacillus prodigiosus, called «Coley's Toxins,» to treat tumors.

Lane 1: Wild - type HAP1 whole cell lysate (20 µg) Lane 2: IKB alpha knockout HAP1 whole cell lysate (20 µg) Lane 3: Hela whole cell lysate (20 µg)

Immunoprecipitates and cell lysates were separated by SDS — PAGE followed by western blotting.

Lysates were immunoblotted for protein levels of (A) phosphorylated ERK (pERK), total ERK (tERK), and β - actin or (B) phosphorylated IκBα (p - IκBα), total IκBα (IκBα), and β - actin.

Rat brain tissue lysate was subjected to SDS PAGE followed by western blot with 23449 -1-AP (UBQLN2 antibody) at a dilution of 1:800 incubated at room temperature for 1.5 hours.

ab32518 (purified) at 1/20 immunoprecipitating IKB alpha in HeLa cell lysate (Lane 1).

As seen in in vitro co-culture studies, EphA2, EphB2 and EphB4 phosphorylation was reduced in whole tumour lysates of Tie2PEKO animals, independently of changes in gene expression (Supplementary Fig. 10).

Normalized protein lysates were subjected to SDS - PAGE, transferred to nitrocellulose, and immunoblotted with primary Abs from Cell Signaling Technology (Danvers, MA) at concentrations recommended by the manufacturer.

Cell lysates were incubated with protein G sepharose beads (GE Healthcare) and 3 μg Tie2 antibody (Millipore, clone Ab33, # 05 - 584) overnight at 4 °C on a rotator.

Calpain inhibitor (1 μl) added to treated cell lysate was used as a negative control.

The Clinical Biomarkers Facility offers services for high - throughput and highly specific analyses of protein biomarker candidates in body fluids such as plasma, serum, cerebrospinal fluids etc. and cell and tissue lysates using molecular tools such as proximity extension and proximity ligation technologies (PEA and PLA) providing assays with high specificity and sensitivity in complex biological matrices.

Co-culture of Tie2 - silenced pericytes with EC resulted in reduced EphA2, EphB2 and EphB4 phosphorylation in the co-culture lysates (Fig. 2h).

Unpurified ab32518 used to immunoprecipitate IKB alpha from human HeLa whole cell lysate.

Unpurified ab32518 used to immunoprecipitate IKB alpha from rat PC12 whole cell lysate.

Subsequently, lungs were digested with DMEM containing 1.25 mM CaCl2, 200 U ml − 1 collagenase I and 10 μg ml − 1 DNase I at 37 °C for 1 h. Single - cell suspensions of the digested organ were prepared by passing through 18G and 19G cannula syringes and filtering the lysates through a 100 μm cell strainer.

WB: Jurkat whole cell lysate.

Total cell lysates of HuH - 7 cells were incubated with GST or GST - Sp1 fusion protein — prebound glutathione - Sepharose 4B beads and the bound fractions were subjected to immunoblotting with antibody against DDX3 (top).

Aβ 1 to 40 and 1 to 42 levels in hippocampal lysate were analyzed using the Aβ1 - 40 and Aβ1 - 42 ELISA kits from Invitrogen (# KHB3481 and # KHB3442, respectively).

Lysates from these two cell populations were fractionated on the basis of the subcellular localization using the ProteoExtract Kit, and 40 μg of proteins was loaded for Western blotting.

Tip 3: Make sure protease inhibitors are present in the lysate buffer.

The concentration of protease inhibitor should be 1.5 — 2 times that of a typical lysate preparation protocol for Western blotting.

The assay has been validated for use with human and mouse cell lysates and tissue homogenates, such as peripheral blood mononuclear cell (PBMC) lysates, and will be marketed through Enzo's worldwide sales and marketing group.

Cell lysates of hippocampal tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).

Whole - cell lysates were prepared 24 and 48 hours following treatment.

Cell lysates of either hippocampal tissue or entorhinal cortex tissue from aged AD mice on control diet (AD Ctl) or J147 diet (AD J147) were analyzed by Western blotting and the images quantified in bar graphs accompanying the images.

Whole - cell lysates were prepared from control and infected cells 48 hours after infection.

For the coimmunoprecipitation experiments, HuH - 7 cells transfected with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].

Live cell assays constructed with genetically encoded, fluorescent biosensors can provide significant advantages over endpoint assays measured in cell lysates because functional information about the timing and location of cellular responses can be monitored in cells that are relevant to disease.

Karpas 299 and SU - DHL1 cells were transiently transfected with 20 μg mTOR siRNA and whole - cell lysates were prepared at 48 hours.

(E) Immunoblots were performed on whole - cell lysates (pSTAT5, STAT5, and tubulin) or conditioned medium (MMP1, IL - 6, and TNC) of parental 1833 and SCP28 cells.

Antibodies with an uncertain routine WB have been revalidated using an over-expression lysate (VERIFY Tagged Antigen (TM), OriGene Technologies, Rockville, MD) as a positive control.

All genes with Data reliability «Supported» in one or both of the two methods immunohistochemistry and immunofluorescence, or standard validation «Supported» for the Western blot application (assays using over-expression lysates not included) are classified as «Evidence at protein level».

(I) Immunoblotting was performed using the indicated antibodies on whole - cell lysates from cells incubated or not with TRAIL.

Cell lysates were harvested and subjected to immunoblot analysis for MEK1, MEK2, MKK3, MKK6, phosphorylated ERK1 / 2 (p - ERK1 / 2), total ERK1 / 2 (t - ERK1 / 2), phosphorylated p38 (p - p38), total p38 (t - p38) and actin.

(K) Immunoblots were performed on whole - cell lysate.

Cell lysates were centrifuged at 15,000 rpm for 5 min.

Cell lysates were subjected to western blotting and sequentially probed with Abs to phosphorylated p38 (phospho - p38), total p38 or actin.

Lysates were sonicated using a Fisher Scientific Sonic Dismembrator (Model 500) at 35 % power for 30 seconds on ice and then boiled for 10 minutes.