Sentences with phrase «anti-hiv lentiviral vector»
The study also examines the use of lentiviral vectors for cell delivery in treating liver diseases, a tool traditionally used in treating blood disorders.
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Germline Transmission and Tissue - Specific Expression of Transgenes Delivered by Lentiviral Vectors
Single - cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter.
This is the first published report of lentiviral vector mediated TCR therapy that has shown persistence beyond one month, June said.
The treatment involves collected blood stem cells carrying the genetic anomaly of patients and corrected them in the laboratory by introducing a healthy WAS gene using a lentiviral vector developed and produced by Genethon.
OMC also aims at potentiating the existing Conditional Mutagenesis Facility by implementing novel approaches to attain tissue -, stage - and site - specific transgene expression in mouse mutant strains, mainly through lentiviral vector - mediated transduction of blastocysts.
Lombardo A, Genovese P, Beausejour CM, Colleoni S, Lee YL, Kim KA, Ando D, Urnov FD, Galli C, Gregory PD, Holmes MC, Naldini L. Gene editing in human stem cells using zinc finger nucleases and integrase - defective lentiviral vector delivery.
Targeting vascular injury using Hantavirus - pseudotyped lentiviral vectors.
Cronin J, et al. «Altering the tropism of lentiviral vectors through pseudotyping.»
Cronin, J., Zhang, X.Y., Reiser, J. «Altering the tropism of lentiviral vectors through pseudotyping.»
Many times lentiviral vectors are engineered with non-native envelopes, a process called pseudotyping.
A method to assess target gene involvement in angiogenesis in vitro and in vivo using lentiviral vectors expressing shRNA.
«The significance of controlled conditions in lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer events.»
VCN and RCL Testing for Lentiviral Vectors Lindsay Skrdlant, PhD, Stanford University School of Medicine, United States
Functional KL2 genes were delivered to the mice using lentiviral vectors, based on a crippled version of HIV that was first developed by Verma and Salk Institute colleagues in 1996.
Integrase - defective lentiviral vectors: progress and applications.
Lentiviral vectors are promising agents for cancer immunotherapy, but this research is still in the pre - clinical / early clinical stages.
for applications that require the large genetic capacity of a lentiviral vector but for which transient transgene expression is sufficient.
lentiviral vectors contain a deletion in the 3» LTR that prevents aberrant activation of nearby genes.
But what's more, the transplanted cells keep producing a foreign protein slipped inside with the help of a lentiviral vector, the kind usually used for gene therapy.
Lentiviral vectors are incredibly popular in the lab - the Trono lab's lentiviral packaging and envelope plasmids are two of Addgene's most requested plasmids!
These images show the development of cancer (dark purple) in the mouse lung, initiated by lentiviral vector.
Researchers are also working to make lentiviral vectors safer.
In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector.
Read on to learn more about the current (and future) applications of lentiviral vectors!
Lentiviral vectors are also commonly used to make stable transgene - expressing or knockout cell lines.
Review of «Safety and efficacy of a tCD25 pre-selective combination anti-HIV lentiviral vector in human hematopoietic stem and progenitor cells» from Stem Cells by Stuart P. Atkinson
Preintegration HIV - 1 inhibition by a combination lentiviral vector containing a chimeric TRIM5 alpha protein, a CCR5 shRNA, and a TAR decoy.
Kim Y - S, Wielgosz M, Hargrove P, Kepes S, Gray J, Persons D, Nienhuis A. Transduction of Human Primitive Repopulating Hematopoietic Cells with Lentiviral Vectors Pseudotyped with Various Envelope Proteins.
Lentiviral vector technology has come a long way since the 1990s, both in terms of research impact and safety.
The vast majority of the immune cells, including 100 percent of the T and natural killer cells, carried the normal gene that was carried in the lentiviral vector.
A lentiviral vector with the same modifier, Fuw - dCas9 - Tet1CD, is available from Rudolf Jaenisch's lab in plasmid form or as ready - to - use lentivirus.
In 2003, VIRxSYS began the first clinical trial with a lentiviral vector.
The library consists of a pool of lentiviral vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target cells» genomes upon infection.
CD4 + T - cells from patients with HIV - 1 were transduced with a lentiviral vector containing an antisense sequence against the HIV - 1 envelope.
Suppression of clonal dominance in cultured human lymphoid cells by addition of the cHS4 insulator to a lentiviral vector.
Clinical benefits of lentiviral vectors have been observed in small trials for other diseases, including the common hematopoietic disorders
Although adeno - associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more genetic cargo (8 kb vs 4.5 kb), and they remain popular for targeting and tracing cells in the brain (Figure 1).
The Gibco ™ LV - MAX ™ Lentiviral Production System is the first system that provides a scalable and high - yield lentiviral vector production on a serum - free suspension platform, which facilitates the research from bench to clinic.
Efficient gene transfer into rhesus repopulating hematopoietic stem cells using a simian immunodeficiency virus - based lentiviral vector system.
High level erythroid lineage - directed gene expression using globin gene regulatory elements following lentiviral vector mediated gene transfer into primitive human and murine hematopoietic cells.
Generation of an HIV -1-resistant immune system with CD34 (+) hematopoietic stem cells transduced with a triple - combination anti-HIV lentiviral vector.
Researchers have also developed integrase - deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector but for which transient transgene expression is sufficient.
Since lentiviral vectors can deliver a large amount of DNA (~ 8 kb) with a relatively low immune response, it should come as no surprise that researchers are interested in developing these vectors for gene therapy.
Mobilization and mechanism of transcription of integrated self - inactivating (SIN) lentiviral vectors.
To sidestep the shortcomings of currently used cancer models, the Salk team harnessed the power of lentiviral vectors to infect nondividing as well as dividing cells and ferry activated oncogenes into a small number of cells in adult, fully immunocompetent mice.
Verma has successfully developed lentiviral vector - mediated mouse models for glioblastoma, lung adenocarcinoma and small cell lung cancer (SCLC).
Lentiviral vectors in cancer immunotherapy.
Therapeutic levels of fetal hemoglobin in erythroid progeny of -LCB- beta -RCB-- thalassemic CD34 + cells following lentiviral vector - mediated gene transfer.
Extended ß - globin locus control region elements promote consistent therapeutic expression of a γ - globin lentiviral vector in murine ß - thalassemia.