Sentences with phrase «as microarray»

As the microarray contains a diversity of genes originated from different organisms, all genes were transformed to Homo sapiens ones.

As microarray technologies continue to develop, the chemistry underlying the biology will increasingly come to the forefront.

There are and will continue to be jobs at companies that develop instruments and kits, such as microarrays, reagents, expression kits, and other supplies to fuel research programs.

The completion of a draft genome sequence for the microorganism Methanosarcina barkeri has enabled use of high - throughput technologies such as microarrays and mass spectrometry of proteins to detect global changes that occur in response to experimental treatments.

Not exact matches

The panel can be used as a tool kit for screening and profiling of functional lncRNAs, or as an accurate quantification to validate functional lncRNAs in microarray or RNA - sequencing data.

Larger biomarker signatures can be detected with technology from CDI Laboratories, which offers microarrays of functional human proteins (over 20,000 on a single array) to test the antibodies present in human liquid biopsy samples, such as blood, serum, plasma, CSF, or tissue lysates.

As ICREA Prof. at ICFO Valerio Pruneri comments «the device means a major step forward for light microscopy techniques, especially for microarray platforms since it could definitely be used as a point - of - care tool in the diagnosis and treatment of major diseases such as Sepsis, a critical area where fast and accurate results can translate into life changing health outcomes for individualAs ICREA Prof. at ICFO Valerio Pruneri comments «the device means a major step forward for light microscopy techniques, especially for microarray platforms since it could definitely be used as a point - of - care tool in the diagnosis and treatment of major diseases such as Sepsis, a critical area where fast and accurate results can translate into life changing health outcomes for individualas a point - of - care tool in the diagnosis and treatment of major diseases such as Sepsis, a critical area where fast and accurate results can translate into life changing health outcomes for individualas Sepsis, a critical area where fast and accurate results can translate into life changing health outcomes for individuals.

One of these, his most influential, outlines his original use of microarray expression profiles as a phenotypic footprint for gene function.

Microarrays have revolutionised functional genomics for biologists in much the same way as the microprocessor revolutionised the world of computing science.

Working at a microarray core facility is not where many of us thought we would be today, simply because microarrays were not as popular (or even around!)

Large - scale methods of probing samples, such as DNA sequencing, microarrays, and automated gene - function studies, are filling new databases to the brim.

In the relatively young field of microarrays, technical positions are never mundane or boring, because there are always opportunities to evaluate and refine new techniques and protocols as they become available.

The University Health Network's Microarray Centre was established as part of a consortium of research institutions in Toronto.

As novel applications, such as drug screening, SNP detection, diagnostics, environmental monitoring, and food protection, are developed, there will be more demand for experienced microarray technicians in the workplacAs novel applications, such as drug screening, SNP detection, diagnostics, environmental monitoring, and food protection, are developed, there will be more demand for experienced microarray technicians in the workplacas drug screening, SNP detection, diagnostics, environmental monitoring, and food protection, are developed, there will be more demand for experienced microarray technicians in the workplace.

During our undergraduate and graduate training in the mid-1990's, microarrays, as we know them today, were not widely used.

He's excited to see a clear readout for a process that would otherwise require decoding a complex pattern on a microarray, and he thinks the idea of simple alphanumeric display could be applied to other types of molecular logic as well.

«The genetic content that goes on a microarray is as important as the technology itself,» he says.

As a research associate in the pathology labs of Yale School of Medicine, Mrs. Canosa has worked extensively with microarray expression profiling, genotyping, and DNA sequencing.

«We tried to do a thorough study,» Scherer says, «so that we could convince people to do a microarray analysis as part of their workup.»

Melton and colleagues are now doing large scale screening with microarrays to track gene activity as cells develop and discover gene products that move cells along the proper paths.

We have compared the transcriptome in blood leukocytes, liver, and brain of humans, chimpanzees, orangutans, and macaques using microarrays, as well as protein expression patterns of humans and chimpanzees using two - dimensional gel electrophoresis.

He is on the Board of Directors of the Cancer Cytogenomics Microarray Consortium and reviews manuscripts for many top journals in his field as well as having over 150 peer - reviewed publications.

Periostin (POSTN) was identified by microarray RNA profiling studies as a stromal factor involved in maintaining the normal stem cell niche and demonstrated to be secreted by stromal fibroblasts, but not by infiltrating tumor cells (Malanchi et al., 2012).

The Virochip, a pan-viral microarray containing ∼ 19,000 probes derived from all viral species in GenBank (n ∼ 2500)[21], [23], has been previously successful in detection of novel outbreak viruses such as the SARS coronavirus [22], [24] and the 2009 pandemic H1N1 influenza virus [23].

Here we applied the Virochip, a pan-viral microarray, to identify a novel adenovirus (TMAdV, titi monkey adenovirus) as the cause of a deadly outbreak in a closed colony of New World monkeys (titi monkeys; Callicebus cupreus) at the California National Primate Research Center (CNPRC).

For expression analysis in mice, we used microarray data as described above to select two internal control genes, cyclophilin B (Cphn2) and ribosomal protein S3 (Rps3).

Antibody microarrays are powerful tools for simultaneous detection of hundreds or thousands of blood - borne protein biomarkers at once, potentially allowing accurate fingerprinting and improved diagnostics of complex pathophysiological states such as cancers, neurodegenerative diseases and autoimmune conditions.

Using the GOMiner algorithm, two independent category structures (biologic process and molecular function) based on the 1178 rat genes represented on the microarray (of which 923 currently carry GO annotations) were initially constructed and used as Query gene files.

Both firms utilize microarray genotyping to pinpoint hundreds of thousands of single nucleotide polymorphisms (SNPs) that indicate phenotypic traits such as eye color, hair color, and freckling as well as geographic ancestry.

Microarray analysis was performed at different time points to determine yeast genome - wide response when exposed to salt, acid, hypoxic conditions, and hydrogen peroxide, as well as ethanol, isopentenol, and other biofuels candidates.

[NEWS, 4 June 2013] Protein microarrays, representing more than one third of all human proteins, provide a unique possibility to study complex autoimmune diseases, such as multiple sclerosis (MS).

Individual 45 - mer oligonucleotides corresponding to mRNAs of each gene were used as probes, and immobilized on microarray slides, as described below.

The raw data as well as the processed data of the microarray were deposited under Gene Expression Omnibus (GEO)(http://www.ncbi.nlm.nih.gov/geo) accession number GSE87159.

The facility collaborates with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing, microarrays as well as fluorescence insitu hybridisation (FISH) experiment.

The names of samples within the microarray analysis were labeled C11 and T11 as for control (reference) and treatment at daytime (11:00), respectively.

As mRNA isolation is simple and since DNA microarrays are a proven genomics reagent for monitoring differential gene expression, a genome - wide expression analysis may be the fastest and most efficient method for identifying additional candidate effectors of colony queen number.

Clinical Diagnostics The platform will provide service and support in order to promote and adapt the use of high throughput techniques, such as next - generation sequencing, microarray - based analyses and multiplex protein analysis, in routine diagnostics.

Each neoplasm was part of a tissue microarray constructed as described elsewhere (22).

The high resolution that is achieved by these techniques, particularly by microarray technologies such as array comparative genomic hybridization, is blurring the traditional distinction between cytogenetics and molecular biology.

In addition, analysis of the microarray results obtained with RNA from the optimized protocol showed that, as expected, the genes involved in methane production (methanogenesis) were among the most highly expressed genes in M. barkeri.

Microarray analysis revealed temperature - dependent expression of distinct sets of genes, as well as 700 A. fumigatus genes not present or significantly diverged in the closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenicity phenotype.

To reveal the process as to how this occurs, the researchers created the first full - mouse genomic platform of DNA microarrays.

CDI has produced the ZikaProt ZIKV / DENV protein microarray which contains the proteomes of Asian and African strains of Zika as well as dengue serotypes 1 and 2.

Research on vaccine biomarkers, including in - depth comparative analysis of data from different platforms, large - scale RNA sequencing, harmonisation of standard operating procedures (SOPs) for sample, microarray and data analysis, as well as transcriptome mapping (more than 1400 samples analysed).

However, when the antibody is evaluated on a denatured microarray (above) it is no longer specific for p53, and is, in fact, monospecific for C20orf77 as the S - Score (between rank # 1 and rank # 2) is 9.55.

Quantitative real time PCR (qRT - PCR) is accepted as the gold standard for expression profiling, so we next compared both our RNAseq and the microarray expression estimates to a panel of qRT - PCR TaqMan gene expression assays (Figure 2C — F).

Dr. Li's research interests focus on machine learning, bioinformatics, and statistical data mining in large scale data in biomedical research, such as next generation sequencing data (whole genome sequencing, RNA - seq, microarray data), in the file.

Microarray profiling identified STAT6 as one of the proteins whose expression was increased in apoptosis - resistant cells (Gagarin et al., 2005).

By directly comparing the gene expression estimates by these two techniques, using a correlation with TaqMan qRT - PCR as a benchmark, we find RNAseq provides measurements over at least two orders of magnitude greater than the microarray, and thus correlates better with TaqMan values across a large dynamic range.

While there are techniques to identify known viruses, such as high throughput sequencing or high density microarrays, identifying truly novel virions could pose a problem, the authors write.