Sentences with phrase «b cells expressing»

In another experiment, Kelsoe found that B cells expressing the rearrangement genes bound less tightly to an antigen than did B cells that didn't rearrange their DNA.

Chronic lymphocytic leukemia B cells express functional CXCR4 chemokine receptors that mediate spontaneous migration beneath bone marrow stromal cells.

Not exact matches

Since genes for the T - cell receptor beta chain were previously shown to be on mouse chromosome 6, all three of the Ig - like multigene families expressed and rearranged in T cells are located on different chromosomes, just as are the B - cell multigene families for the Ig heavy chain, and the Ig kappa and lambda light chains.

Functional glutamate receptor (GluRs) were transiently expressed in cultured mammalian cells from cloned complementary DNAs encoding GluR - A, - B, - C, or - D polypeptides.

This clinical trial sponsored by Juno Therapeutics is testing JCAR017, which targets the CD19 protein expressed on most B - cell leukemias and lymphomas.

In PLAT - 02, the CAR T cells are reprogrammed to recognize and target the CD19 protein that is expressed by most precursor B acute lymphoblastic leukemia cells.

But rather than delivering the entire gene for the clotting - factor proteins to cells, as most gene therapies do, the researchers used the viruses to engineer immune - regulating B cells to express a fragment of the clotting factor fused to an immune molecule called an immunoglobulin.

Thus, this cDNA derives from a gene (oct - 2) that specifies an octamer binding protein expressed preferentially in B lymphocytes, proving that, for at least one gene, a cell - specific transcription factor exists and its amount is controlled through messenger RNA availability.

Of course, it makes sense that viruses would choose to turn off genes that the immune system needs to fight the virus, «like interferon - b, which is a highly anti-viral gene expressed in virtually all cell types; or genes that T cells need to recognize virus - infected cells,» Kuss - Duerkop says.

Consistent with that result, the researchers found that certain regions of the X chromosome in human female B cells, including regions that contain immunity - related genes, were expressed at higher levels than male cells.

These are images of mouse embryonic stem cells which grow in a round colony of cells (A) and express Sox2 (B), shown in red.

Dsg3 CAAR - T cells exhibit specific cytotoxicity against cells expressing anti-Dsg3 BCRs in vitro and expand, persist, and specifically eliminate Dsg3 - specific B cells in vivo.

When given to diseased mice, the engineered T cells targeted and killed B cells that express antibodies targeting desmoglein 3, hinting that such a strategy may be an effective way to treat antibody - driven autoimmune diseases.

The current study showed that GARP was expressed upon activation of B cells and may therefore act as an important checkpoint for B cell tolerance.

«If you look at GARP expression on B cells, usually it's only expressed on activated B cells in the periphery.

«Role of RNA binding protein in driving cancer, leukemia study reveals: Abnormally expressed in cancer cells, the protein was found to promote the proliferation of B cells in B - cell acute lymphoblastic leukemia.»

As Hayday explains, his team looked at myriad genes expressed in blood samples, most of which were not related to B cells.

The fraction of F4 / 80 - expressing cells was greater in moderately obese mice (b and e) and greatest in the severely obese Lepob / ob mice (c and f).

The adipocyte - enriched population (gray bars) expressed small amounts of the macrophage markers (a), consistent with residual macrophage contamination seen by immunofluorescent staining of live cells (b).

We observed similar robust increases of intracellular calcium concentrations in response to KCl and ATP in both GFP - expressing (n = 28) and GFP - nonexpressing cells (n = 12), whereas 5 - HT elicited an increase in intracellular calcium only in GFP - expressing cells (KCl: t (40) = 0.98, p = 0.33; ATP t (38) = 1.04, p = 0.30; 5 - HT: t (38) = 5.24, p < 0.0001 Student's t tests; Fig. 4A, B, F).

Immunohistochemical detection of cells expressing the macrophage - specific antigen F4 / 80 (arrows) in extensor digitalis longus muscles from C57BL / 6J (a and c) Lepob / ob female and (b and d) lean female mice.

B, Histograms of GFP and P2X3 normalized immunoreactivity of individual cells showing that whereas the P2X3 staining shows a unimodal normal distribution (mean 0.58), the GFP population is bimodal suggestive of high - expressing (mean = 0.69) and low - or nonexpressing populations (mean 0.12).

To determine whether adipose tissue macrophages express any molecules implicated in obesity - associated complications, we isolated three cell populations from the parametrial adipose tissue of three obese B6.V Lepob / ob mice: (a) an adipocyte - enriched population, (b) a stromal vascular macrophage F4 / 80 + population, and (c) an F4 / 80 — stromal vascular population.

Blincyto (blinatumomab) is designed to treat cancers expressing a molecule called CD19 — found on the surface of B cell ALL and also non-Hodgkin's lymphoma.

Deciphering the role in cancer of «non-conventional» T cells that recognize lipid antigens presented by CD1 molecules, non-polymorphic MHC - I - related molecules expressed by mature myeloid and B cells.

(B) In contrast, EFTF / YFP - expressing cells generate mosaic retinas with columns of cells spanning the entire retinal width.

(PMID: 9312132) Download PDF [ii] Wong BR, Josien R, Lee SY, Sauter B, Li H - L, Steinman RM, Choi Y. TRANCE, a new TNF family member predominantly expressed in T cells, is a dendritic cell specific survival factor.

Memory CD4 T cells that express CXCR5 provide accelerated help to B cells.

Notch ligands expressed by follicular dendritic cells protect germinal center B cells from apoptosis.

The enhanced hiPS - HEP cells respond to insulin with phosphorylation of protein kinase B - α (Akt), even at low insulin concentrations, and the genes involved in glycogen metabolism, gluconeogenesis, and insulin signaling are expressed at similar levels as in hphep cells.

The researchers particularly identified three novel candidate genes; KIRREL3 and SLIT1, with documented roles in immune cell development, and SERPINA9 expressed exclusively at the site (germinal center) where a B - cell starts producing IgA.

Albumin and α1AT are well - expressed in enhanced hiPS - HEP cells from all three hiPS cell lines (Figure 3, Panels A, B, and C), as shown by immunostaining and mRNA expression analysis.

Furthermore, enhanced hiPS - HEP cells express high levels of the LDL receptor (Figure 5, Panel B) and take up fluorescently labeled LDL (Figure 5, Panel A).

CD11c - Expressing B Cells Are Located at the T Cell / B Cell Border in Spleen and Are Potent APCs.

Other genes relevant for regulating blood cholesterol levels are well - expressed in enhanced hiPS - HEP cells, e.g., apolipoprotein B (a VLDL), apolipoprotein A1 (an HDL), PCSK9, sterol regulatory element - binding proteins 1 and 2 (SREBP - 1 and -2), and lipoprotein lipase (LPL), which hydrolyzes triglycerides in lipoproteins into free fatty acids and glycerol.

Here, we show that enhanced hiPS - HEP cells respond to insulin with phosphorylation of protein kinase B - α (Akt), even at low insulin concentrations (Figure 4, Panels E and F), and that the genes involved in glycogen metabolism, gluconeogenesis, and insulin signaling are expressed at similar levels as in hphep cells (Figure 4, Panels B, C, and D).

Further, consistent with T cell phenotypes in nontumor - bearing mice (Fig. 2), we observed an increased percentage of spleen - derived CD8 + T cells expressing high levels of the activation marker CD44 in DGKζ − / − or DKO mice that had been inoculated with tumor when compared with Cbl - b − / − or WT mice (Fig. 4C).

CD20 is a B - lymphocyte surface molecule that is widely expressed during B - cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells.

DEC1 / STRA13 is a key negative regulator of activation - induced proliferation of human B cells highly expressed in anergic cells

Both normal B cells and the cancerous B cells from which some lymphomas arise express CD19.

Both normal B cells and the cancerous B cells from which leukemias arise express CD19.

We describe in this study anti-BTLA mAbs that demonstrate that the protein is expressed in the bone marrow and thymus on developing B and T cells, respectively.

(A, B) Culturing wild - type brains with conditioned media from GBP1 and GBP2 expressing cells induces low level of ILP2 (A) and ILP5 (B) accumulation in the insulin - producing cells.

(B) Representative percentage of donor - derived spleen T cells expressing Foxp3 on days 7, 14, and 28.

(B) Representative images of cells expressing HMOX1 following compound treatment.

(B) In situ hybridization reveals Lcn16 is expressed in glandular tissue of the VNO and (C) Lcn17 is expressed in cells within the main olfactory epithelium.

The lab chose to use the TCL1 mouse model to study B - cell leukemia because ~ 90 percent of human chronic lymphocytic leukemia (CLL) patients express the TCL1 protein, and the overexpression of TCL1 in B cells leads to the development of CLL in mice.

(A, B) Neurite length of neurons in E14 rat VM cultures transfected with control siRNA, Smad4 siRNA or Smad1 siRNA or Smad5 siRNA and a GFP - expressing plasmid before being cultured with or without 10 ng / ml GDF5 for 72 h (*** P < 0.001 compared with control; ANOVA with post-hoc Tukey's test; 40 cells for each group per experiment; number of repetitions (n) = 3 experiments).

(A) Neurite length and (B) neurite branching of SH - SY5Y cells transfected with control siRNA or Smad1 siRNA or Smad5 siRNA and a GFP - expressing plasmid together with a control or miR - 181a antagomir for 72 h (* P < 0.05, *** P < 0.001 compared with control)(# P < 0.05, ## P < 0.01, ### P < 0.001 ANOVA with post-hoc Tukey's test; 40 cells for each group per experiment; n = 3 experiments).

B was expressed throughout the adult central nervous system with high efficiency in most cell types,» says Gradinaru.