Sentences with phrase «b cells used»

Tonsillar lymphocytes were depleted of CD19 + B cells using the eBioscience Magnisort CD19 positive selection kit.

For sorting human ILCs, tonsillar lymphocytes were depleted of B cells using CD19 magnetic beads (eBioscience).

By targeting antigens to B cells using monoclonal antibodies, he successfully inhibited the development of multiple sclerosis in an animal model.

Developing B cells use somatic hypermutation (SHM) to diversify their immunoglobulin (Ig) genes.

Not exact matches

Folic acid is a B vitamin which our bodies use to make new cells.

Breech Twins and higher order multiples Previous CS Pre-Eclampsia Placenta praevia Cervical incompetence Previous late stillbirth Previous premature birth Grand multiparty Age under 18 Age over 35 Smoking Drug use Severe mental health issue Epilepsy Type 1 diabetes Type 2 diabetes Gestational diabetes Asthma GBS positive Abnormal antibodies Transplant recipient Congenital heart disease Known foetal abnormality Immunosuppressive medication MS Physical disability Intellectual disability Hypothyroidism Hyperthyroidism Previous shoulder dystocia Previous 3rd or 4th degree tear Sickle Cell anaemia BMI under 18 or over 35 at conception Previous massive PPH APH in current pregnancy HIV / AIDS Hepatitis B or C Active TB IUGR Oligohydramnios Polyhydramnios Child previously removed from custody because of abuse Uterine abnormalities such as uterine septum or double uterus Previous uterine surgery for fibroids Chronic renal problems Hypertension Auto immune condition Previous stroke or blod clot Cancer Domestic violence or abusive home Prisoners Homeless women

(borrowed from Dr Kitty) Breech Twins and higher order multiples Previous CS Pre-Eclampsia Placenta praevia Cervical incompetence Previous late stillbirth Previous premature birth Grand multiparty Age under 18 Age over 35 Smoking Drug use Severe mental health issue Epilepsy Type 1 diabetes Type 2 diabetes Gestational diabetes Asthma GBS positive Abnormal antibodies Transplant recipient Congenital heart disease Known foetal abnormality Immunosuppressive medication MS Physical disability Intellectual disability Hypothyroidism Hyperthyroidism Previous shoulder dystocia Previous 3rd or 4th degree tear Sickle Cell anaemia BMI under 18 or over 35 at conception Previous massive PPH APH in current pregnancy HIV / AIDS Hepatitis B or C Active TB IUGR Oligohydramnios Polyhydramnios Child previously removed from custody because of abuse Uterine abnormalities such as uterine septum or double uterus Previous uterine surgery for fibroids Chronic renal problems Hypertension Auto immune condition Previous stroke or blod clot Cancer Domestic violence or abusive home Prisoners Homeless women

Using the findings from this study, the team has designed vaccine immunogens to selectively trigger the cooperating antibody - producing B cells to cooperate to make broadly neutralizing antibodies in a manner that mimics broadly neutralizing antibody development in natural HIV infection.

B: Well, we were in the midst of experiments aiming to use an animal virus to introduce new genes into human cells and into bacterial cells.

Using a fluorescent tracker molecule, the team began by tracking the location of Xist in developing B cells in female mice.

He is using the virus to cure a rare form of blood cancer called EBV lymphoma, caused when B lymphocyte immune cells get infected with the Epstein - Barr virus (EBV).

The researchers used their new method to identify a number of previously unknown genes that are involved in B - cell development.

To test their theory, Xiaoli Guo, a post-doctoral researcher in Mitchum's lab and first author of the study, synthesized the CLE - B nematode peptide and applied it to the vascular cells of Arabidopsis, a model plant system used in plant research.

But rather than delivering the entire gene for the clotting - factor proteins to cells, as most gene therapies do, the researchers used the viruses to engineer immune - regulating B cells to express a fragment of the clotting factor fused to an immune molecule called an immunoglobulin.

But in truth, most (26 of 28 vaccines currently licensed for human use) stimulate primarily a B - cell or antibody response, which in many cases is sufficient.

In the study, researchers used the blood of seven people who survived Ebola Bundibugyo virus infection during the 2007 outbreak in Uganda to isolate a large number of B cells that produce antibodies, which are the small protein molecules capable of inactivating the virus.

Westin launched his campaign last spring, and by the fall he had handed over management of his clinical trials — most recently testing the efficacy of a combination of three drugs used to treat large B - cell lymphoma — to colleagues.

In a paper published in the journal Proceedings of the National Academy of Sciences, Sangeeta Bhatia of MIT and Charles Rice of Rockefeller University describe using microfabricated cell cultures to sustain hepatitis B virus in human liver cells, allowing them to study immune responses and drug treatments.

B cells, cytotoxic T cells, and helper T cells boost their use of glucose and increase their reliance on the glycolysis pathway to digest the sugar.

The team used gene expression profiling and found that canine B - cell lymphoma expression profiles were similar in many ways to human B - cell lymphoma, thus paving the way for future studies, including therapeutic clinical trials in dogs and humans.

In experiments using enzyme inhibitors, the possibility that cinnamtannin B - 1 enhanced mobilization through the use of activation of enzymes such as PI3 kinase near the cell membrane was shown.

A new technique of direct binding of radioactive DNA was used to screen a complementary DNA expression library from the BJAB cell line in lambda gt11 phage to derive molecular cDNA clones representing a putative B lymphocyte - specific octamer binding protein.

The probe also has no apparent toxicity in most mammalian cells, so it can be used to monitor in vivo MAO - B activities during various stages of the disease.

The new technique, pioneered by Wilson and fellow researchers at the Emory University School of Medicine in Atlanta, saves time by using antibodies produced by so - called B cells (white blood cells that produce and then ferry them to infection sites to battle invading germs) in response to vaccines instead of to actual infections.

Using mice deficient in Del - 1, they found that the protein promotes proliferation and differentiation of hematopoetic stem cells, sending more of these progenitor cells down a path toward becoming myeloid cells, such as macrophages and neutrophils, rather than lymphocytes, such as T cells and B cells.

One way that a2M might perform this custodial chore is by carrying the bound b amyloid into cells where it can be degraded — a step that requires that it bind to the very same receptor that another protein, called apoE, uses to enter cells.

Selective immunoproteasome inhibition using the inhibitor ONX 0914 reduced the number of B cells and plasma cells and suppressed donor - specific allo - antibody production.

His team has previously shown that using a cancer drug to wipe out immune cells called B - cells in CFS patients seems to relieve the condition.

This can be used to create batches of cells, called UCART19, for treating anyone with the particular form of leukaemia that Layla had (B - cell acute lymphoblastic leukaemia).

The researchers are using the «Proximity Ligation Assay» method to measure the distances between B cell receptors on the surface of the cell.

B cell signaling pathways are the current targets of several therapies used to treat B cell malignancies such as CLL.

The mechanism used by Notch to regulate Myc in B cells is distinct from the mechanism used in other cell types, such as T cells, where Notch also regulates Myc.

His group uses genome - wide and gene - specific DNA methylation analysis to identify aberrantly silenced tumor suppressor genes in B cell leukemias and lymphomas.

About two decades ago, Lerner's laboratory developed some of the first techniques for using B - cell gene - mixing to generate very large «libraries» of antibodies and swiftly determine which of these can bind to a desired target.

Use of exon - based transcriptome profiling to identify novel signaling pathways and survival - associated genes in diffuse large B - cell lymphoma.

The FDA approves Blincyto (blinatumomab) for use in the treatment of B cell acute lymphoblastic leukemia (ALL).

Important to these studies was our development and characterization of a polarized hepatic cell line, called WIF - B, which is now being used widely.

In the study, vitamin B - 6 also was used to treat the damaged RPE cells producing healthy cells that functioned normally.

Now as an Assistant Professor in Medicine at Harvard Medical School, Daniel uses membrane structure and function as a means to define B cell antigen recognition and inform vaccine design.

Using subjects selected by Dr. Susan Levine (Manhattan, NY), Dr. Rita Shaknovich's lab (Weill Cornell Medical School) has purified B, T, and NK cells for preparation of mRNAs, which will be characterized through Illumina sequencing of cDNA libraries at the Cornell Sequencing Facility and analyzed by Dr. Fabien Campagne's group (Weill Cornell Medical School).

This will include testing the mechanism of action of different adjuvant and antigen formulations by measuring B - and T - cell responses and by using various functional assays (ELISA, FACS, cytokine profiles), transcriptomics and / or proteomics.

The HZI will develop a vaccination protocol for mucosal administration based on three novel strategies: (i) development and optimization of a vaccination protocol in which parenterally - primed T and B cells are subsequently pulled into the mucosa by the local delivery of the cognate antigen to the requested effector site, (ii) testing the co-administration of antigens with novel mucosal adjuvants using different mucosal immunisation routes and schedules, and (iii) testing various nanoparticles co-administered with different immunomodulators for their ability to generate both systemic and mucosal immune responses following transcutaneous / trans - follicular vaccination.

As a postdoc, Taylor developed an innovative laboratory technique using metallic additives and magnets to test experimental vaccines to see which B cells bind to them.

Coligation of the B cell receptor with complement receptor type 2 (CR2 / CD21) using its natural ligand C3dg: activation without engagement of an inhibitory signaling pathway.

«We compiled a list of the proteins we thought Zika and similar viruses like dengue were using to get inside of cells, and then we looked to see which of these proteins were abundantly present in the different cell types of the developing brain,» said Pollen, who did the work with Tomasz Nowakowski, PhD, and a UCSF graduate student Carmen Sandoval - Espinosa, BS, both in Kriegstein's lab.

GigaGen captured millions - diverse DNA libraries from B cell and plasma cell repertoires from human donors and used these libraries to manufacture recombinant IgG proteins.

Used as the parental line in deriving B - THP - 1 / DC - SIGN cells (5013) Negative control line used in DC - Used as the parental line in deriving B - THP - 1 / DC - SIGN cells (5013) Negative control line used in DC - used in DC - SIGN

As strain B95 - 8 was used to transform B cells to obtain LCLs, it is always present, but a specific deletion in its genome sets it apart from natural EBV strains.

Cell tracking used 2 - dimensional time - lapse microscopy to monitor 70 cells from each MSC population over 24 hours, with video software able to differentiate between slow (Figure A) and fast (Figure B) cell populations (Each colored line represent the track of a single ceCell tracking used 2 - dimensional time - lapse microscopy to monitor 70 cells from each MSC population over 24 hours, with video software able to differentiate between slow (Figure A) and fast (Figure B) cell populations (Each colored line represent the track of a single cecell populations (Each colored line represent the track of a single cellcell).

To assess granzyme B production, CFSE - labeled cells were stimulated for 24 or 72 h, surface - stained as described above, permeabilized using a Cytofix / Cytoperm kit (BD Pharmingen), and then stained with flurochrome - labeled granzyme B Ab (GB11; Life Technologies) at 4 °C for 30 min prior to flow cytometric analyses.