Sentences with phrase «bd transduction»
Protein - DNA complexes were then immunoprecipitated overnight with polyclonal Oct4 antibody (sc - 9081, Santa Cruz Biotechnology), a mix of monoclonal Oct4 antibodies (BD Transduction Labs, Santa Cruz Biotechnology, Chemicon), or normal serum controls (Pierce) on a rotator at 4 °C.
http://journals.plos.org/
The following primary antibodies were used: Oct4 (BD Transduction Laboratories, 1 ∶ 1000), phospho - p38 MAPK and total p38 MAPK (Cell Signaling), β - Actin clone AC - 15 (Sigma Aldrich, 1 ∶ 5000) and GAPDH (Ambion, 1 ∶ 40,000).
The following primary antibodies were used: Oct - 4 (19857 Abcam) 1 ∶ 1000; β - III tubulin clone SDL.3 D10 (T8660 Sigma) 1 ∶ 500; Nestin (611658 BD Transduction laboratories) 1 ∶ 500 and TH (P40101 Pel - Freez) 1 ∶ 500, and as secondary antibodies: Alexa Fluor 594 Goat Anti-Mouse, Alexa Fluor 488 Goat Anti-Rabbit, Alexa Fluor 594 Goat Anti-Rabbit.