Bacterial genomes contain information about how they cause disease and how they resist antibiotics.
Not exact matches
The cost difference is due, in part, to the smaller scale of the MyMicrobes operation, and in part to the size of the
bacterial genome, which Bork says
contains around 5 billion letters of DNA, compared to the 3.3 billion in the human
genome.
To gain access to the gene -
containing portion of the barley
genome at high resolution, Close and his team identified and sequenced 15,622 BACs or
bacterial artificial chromosomes — small fragments of the barley DNA linked to other DNA to constitute a circular molecule that can replicate and be propagated inside an E. coli
bacterial cell, enabling researchers to produce copies of each BAC for DNA sequencing one small piece of the barley
genome at a time.
Regions with significant
bacterial identity (E value < 1e − 5) were then compared to a second database
containing representative animal
genomes (Homo sapiens, Mus musculus, Rattus rattus, Monodelphis domestica, Gallus gallus, Xenopus laevis, Drosophila melanogaster, Anopheles gambiae, N. vitripennis, A. mellifera, Daphnia magna) to obtain a corresponding «animal» BLASTN E value score.
To identity potential recent LGT events we used BLASTN to compare the D. plexippus genomic scaffolds against a
bacterial database
containing 1,097 complete
bacterial genome sequences downloaded from the National Center for Biotechnology Information (NCBI).