Not exact matches
Diagram of the complete synthetic
signal transduction system in transgenic plants, using TNT as the ligand,
signaling through the
bacterial chimeric histidine kinase, and adapted response regulator, then activating a transcriptional response.
While our analysis does not provide any details about how the computationally re-designed receptor binds the TNT ligand in planta, we consistently see reproducible
signal transduction in our in vivo systems (
bacterial and plant).
We then show that an adapted HK functions in plants by linking it to a transcriptional response through
signal - dependent nuclear translocation of a
bacterial response regulator [12], to produce a synthetic
signal transduction system.
Our synthetic
signal transduction system was built using a modular assembly of
bacterial and plant protein domains.
We previously showed that the
bacterial response regulator PhoB [14], [15] can be adapted to function in a partial synthetic eukaryotic
signal transduction system [12].
Once inside the cell, it can interfere with
bacterial cell
signal transduction and gene transcription processes.