d) Graph showing higher frequency of γH2Ax foci / nuclei at 40 and 80 μM dose of
Bleomycin post silencing in siTRF2 silenced cells compared to Scrambled transfected HCT - 116 cells (p < 0.05).
Not exact matches
d) Immunocytochemistry analysis showing reduced CD44 expression in siTRF2 silenced HCT116
post treatment with
Bleomycin sulphate when compared to scrambled transfected HCT116
post treatment with
Bleomycin.
Low number of γH2Ax foci formed / clonospheric nuclei
post exposure to high concentrations (80μM) of
bleomycin is indicative of rapid repair of DSBs.
a) Graph showing dose dependant (20, 40, 60, 80 µM) increase of number of enriched Side Population of HCT 116
post treatment with
Bleomycin b) Representative FACS analysed FlowJo images showing enriched side population
post treatment with
Bleomycin.
c) Graph showing dose dependant (20, 40, 60, 80 µM) increase in CD44 enriched cells
post treatment with
Bleomycin sulphate (p < 0.001).
d) Graph showing quantitative average olive tail moment in parental and clonospheres
post DNA damage with 40 and 80 μM of
Bleomycin.
We report the presence of cancer stem cell like Side Population and their survival and enrichment
post treatment with
bleomycin in HCT116 cell line.
Comparative DNA damage profile and SP enrichment
post Bleomycin sulphate treatment.