(D) H - 2d - specific allo - CTL lines were established from splenic CD8 - SP cells, and
CTL assay was analyzed for CTL function by using specific (P815) and nonspecific (EL4).
(F) Donor T cells were isolated from recipient spleen on day 14 for
CTL assay against leukemia cells.
FL -
CTL assay: fluorolysometric determination of cell - mediated cytotoxicity using green fluorescent protein and red fluorescent protein expressing target cells.
Performed Inventory and Procurement oversight and assisted and trained all students in activities such as Plasmid Construction, Elutriation of Whole Blood, Dendritic Cell Studies, Organ / Tissue Processing,
Ctl Assays, Cell Culture Maintenance, Transfection, Animal Handling, Flow Cytometry and Microscopy.
Not exact matches
While the individual effects of the drugs on virus - specific
CTL differ somewhat depending on specific
assays, schedules, and doses, treatment with any of the three HDAC inhibitors impaired the ability of
CTL to kill HIV - infected immune cells.
However, in vitro
assays using spleen cells from CD4 KO mice that had rejected BALB / c corneal allografts failed to detect
CTL activity against donor corneal epithelial or endothelial cells.
The FEC pool is useful for QC of ELISPOT,
CTL and Intracellular cytokine
assays.
Spleen cells were isolated 7 — 14 days after C57BL / 6 CD4 KO mice had rejected BALB / c corneal allografts and were tested for anti - BALB / c
CTL in a conventional 4 - hr 51Cr - release
assay using BALB / c corneal epithelial and endothelial target cells.