After three rounds of screening, they identified a single positive cDNA that was recognized by the C3
CTL clone, and its sequence was identical to the mutant Spnb2 protein.
(PMID: 9545200) Dunbar PR, Chen JL, Chao D, Rust N, Teisserenc H, Ogg GS, Romero P, Weynants P, Cerundolo V. Cutting edge: rapid
cloning of tumor - specific
CTL suitable for adoptive immunotherapy of melanoma.