Sentences with phrase «cell sorting by»

See Adhesion functions in cell sorting by mechanically coupling the cortices of adhering cells.

His team applied, among others, techniques of confocal microscopy and cell sorting by flow cytometry which led to the discovery in human muscle biopsies that these myoendothelial cells are located adjacent to the walls of blood vessels.

CD4 + T cells sorted by immunomagnetic beads from splenocytes of animals tolerant to donor - type cardiac allografts were plated at 106 cells / well.

Not exact matches

Internet connectivity is delivered to planes either by expensive ground - based cell towers or via even more expensive satellite relays, which means there will always be a significantly higher cost for this sort of access than there is for the terrestrial equivalent.

If the divine is now used to give the view a supposedly greater philosophical coherence, then I inevitably reach the sort of conclusion implied by Hartshorne's bodily cells with their «little experiences or feelings.»

This depends upon there being a brain, an arrangement of cells in a particular part of the body which by reason of its peculiar coordination makes the given routing able to «know» in a distinctively human manner — quite different from, although certainly continuous with, the sort of «knowing» that is possible for the higher grades of animal life.

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

The experiments included sorting immune cells found at injury sites by molecules on their cellular surfaces, and many hours looking at mouse cells through the microscope.

The exceptional cancer cells that escape the astrocytes do so by producing a protein called Serpin, which acts as a sort of antidote to the death signals fired at them by nearby astrocytes.

CRH, in turn, triggered production of the cell surface protein FasL; trophoblasts expressing FasL then form a protective shield of sorts around the embryo by forcing any attacking immune cells to self - destruct.

«All of the cells of the brain are constantly active and are participating, by a sort of algebraic summation, in every activity.

Depending on which device they buy, researchers can photograph cells at anywhere from 20x to 60x magnification, and use the included software to sort them by purely visual characteristics such as shape and size, fluorescent markers, or both.

You know, just by creating tension points, so the cells have to, sort of, grab onto something, you know, that leads to a certain level of bulking up.

«Previous techniques that have been used to investigate DNA control sequences usually rely on sorting cells one by one and measuring gene activity in each of them,» says Dr Eva Yus, lead author of the paper.

Such methods can be designed to sort cells by size or to identify cells that display a targeted feature, such as a fluorescent dye that has been added.

Viruses technically are not alive, but there is some stage that's sort of in between inert and going through a living stage by hijacking the machinery of cells.

The bone matrix being produced by cells is stained red in samples of cells that are sorted for not expressing ALPL (left), sorted for expressing ALPL (right), and left as an unsorted control (center).

It is a bulk method that sorts different cell populations by tuning their solubility.

Still others gain entry by disguising themselves as the sort of free - floating molecules that our cells routinely gobble up.

Sorting by cell size, the first chip skims off small red blood cells and platelets, letting only CTCs and white blood cells flow past.

«Non-small cell lung cancers can be sorted in clusters by endocytic changes: Sorting cancer by defective endocytosis genes.»

«All sorts of reactions in cells are controlled by modifying proteins with kinases and phosphatases,» said Keating.

Chang got a genetic foothold on this sensory system by labeling GPCRs in mice and engineering them to produce channelrhodopsin, a molecule that allowed the scientists to use light to manipulate the cells» function and study the neurons sorted by different GPCRs.

New research at Rockefeller University, published March 18 in Nature, reveals how cells sort out the loops meant to encode small RNAs, known as microRNAs, by tagging them with a chemical group.

Microfluidic devices are tiny chips that can sort out disease biomarkers, cells and other small structures in samples like blood by using microscopic channels incorporated into the devices.

By tilting the sound source so that it is not perpendicular, the researchers created better separation distance and could more efficiently sort cells.

This disruption halts the maturation of germ cells by interfering with meiosis, the process by which an individual's genetic material is divided and sorted into what eventually becomes eggs and sperm.

Discovering that she could use a fluorescence - activated cell sorting machine to distinguish non-neuronal cells from neurons by making them glow in different colors was «a high point,» she says.

In a paper published Feb. 19 in Nature Cell Biology, the scientists show that a cutting - edge technique called asymmetric flow field - flow fractionation (AF4) can efficiently sort nano - sized particles, called exosomes, that are secreted by cancer cells and contain DNA, RNA, fats and proteins.

Naïve CD4 + T cells were isolated by MACS microbead negative sorting using the naïve T cell isolation kit (Miltenyi Biotec).

Our success in these areas is complemented by our substantial investment in cutting - edge facilities for nuclear magnetic resonance, protein crystallization, X-ray crystallography, transgenics, histochemistry, electron microscopy, confocal microscopy, and fluorescence activated cell sorting.

Rapsyn is made by our muscle cells and considered a sort of biological anchor that interacts with the acetylcholine receptors to ensure that they are optimally positioned for our muscles to receive orders from our brain.

Dr. Zhu said loss of the gene and the protein it expresses may accelerate regeneration by reorganizing how genes are packaged in the genome so that the cells can more easily switch back and forth toward a more regenerative state, sort of like a toggle switch.

GFP - positive cells, indicative of reactivated latent HIV - 1, were isolated by fluorescence activated cell sorting (FACS).

300 μl of ice - cold 1 × binding buffer were then added to each sample and the samples were analyzed by fluorescence - activated cell sorting (FACS) within one hour for maximal signal.

Single cells from this sorted population were then seeded into each well of a 96 - well plate by limiting dilution.

But these cells also continue to be produced by the hippocampus, a part of the brain that helps people store and sort memories, and can move to other parts of the brain.

Investigating the dynamics of cell sorting at compartment boundaries by real - time in vivo imaging

Format: Website Years Covered: currently active UK trials as of June 2017 Search Strategy: Not disclosed Project Focus: advanced cell therapy, active that year, in the UK only Special Notes: on - line sort by 4 parameters, archives for previous years back to 2012.

March 6, 2013 One region, two functions: Brain cells» multitasking may be a key to understanding overall brain function A region of the brain known to play a key role in visual and spatial processing has a parallel function: sorting visual information into categories, according to a new study by researchers at the University of Chicago.

• Flowmetric — Founded by CEO, Ren Capocasale, Flowmetric is a contract research organization focused on providing polychromatic flow cytometry and cell sorting services to bio-pharma and drug discovery companies.

While this situation is bad for researchers, it turns out that the same sort of force may be helpful in cells by forcing the DNA open just a bit to get the ball rolling on transcribing a gene into messenger RNA (mRNA).

By combining advanced approaches such as cell sorting and single cell analysis, researchers will gain a better understanding of the complex genetic landscape of disease.

Berkeley Lab's Len Pennacchio (left) and Axel Visel led the development of a new technique for identifying gene enhancers called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing.

Our results suggest a sorting mechanism by which an increased cell bond tension guides the rearrangement of cells after cell division to maintain straight and sharp compartment boundaries.

However, as demonstrated by a recent report from Stuelten and colleagues [13], the complexity of CSC markers continues to pose challenges for identifying and isolating the putative tumor stem cell populations by the cell - sorting approach.

With the proven success of the imaging technique, Larabell said it's possible to perform statistical analyses based on large collections of cell nuclei images sorted by different stages of development.

(C) Sorted Lin - CD127 + cells from Rag1 - / - splenocytes were pretreated with DMSO or indicated concentrations of PD98059 or SB203580 as in A and analyzed for IL - 22 secretion by ELISA.

(A) Human tonsillar lymphocytes or (B) sorted Lin - CD127 + cells were pretreated with lethal toxin (1 μg / ml) for 2 — 3 hr followed by IL - 23 (50 ng / ml) stimulation for 18 hrs.

«But the brain works by virtue of these connections that allow one nerve cell to talk to many other nerve cells, sort of like a Twitter account, and each nerve cell is also the recipient of a network of information from thousands of other nerve cells.»