Sentences with phrase «cell suspension culture»

Synchronization of Medicago sativa Cell Suspension Culture.

Not exact matches

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

GenIbet offers 1000 m2 of GMP - compliant upstream and downstream manufacturing facilities, including QC labs and R&D facilities, three BSL2 units for the cultivation of bacterial cells (50 L capacity), insect and mammalian cells (50 L capacity for suspension cell cultures, 168,000 cm2 capacity for adherent cells) and the propagation of viruses (50 L capacity, with space to expand up to 200 L).

Microcarriers represent a viable solution by enabling anchorage - dependent stem cells to be cultured in suspension, allowing significant scale - up in bioreactors.

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

Suspension culture of hepatocyte - derived reporter cells in presence of albumin to form stable three - dimensional spheroids.

Immediately after the cells were seeded, each well was checked under the microscope to verify the sparseness of each spherical culture, and only wells containing single - cell suspension with no cell cluster were chosen for tumor - sphere counting after 9 days.

Non-confluent cultures were trypsinized into single - cell suspension, counted, washed with phosphate - buffered saline (PBS), and fixed with 10 % paraformaldehyde.

«Organotypic Explant Culture of Glioblastoma Multiforme and Subsequent Single - Cell Suspension.»

In suspension culture, they formed embryoid bodies (EBs), which contained various cell types originating from three embryonic germ layers.

A single - cell suspension (50 μl) containing 1500 cells was mixed with Matrigel (1:1) and plated on top of the Matrigel base onto wells of a 96 - well plate; 50 μl of complete medium was added, and the cells were cultured for 14 days.

Viral Manufacturing To manufacture viral vectors, two approaches generally are adopted for culturing purposes: scale - out of adherent - cell systems based on 2D planar technologies and scale - up of 3D suspension cell cultures in stirred - tank bioreactors.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

However, the industry appears to favor cells adapted to suspension cultures (e.g., HEK293), which can successfully grow in serum - free media and thus provide a heightened safety profile and reduced cost of goods (CoG)(33).

However, suspension - adapted production cell lines cultured in large - scale bioreactors are more amenable to therapeutic product expression because of their enhanced scalability and elimination of detachment agents, which facilitates regulatory compliance.

UNISI will provide expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a Cytometric bead assay.

Both normal and cancer breast stem cells give rise to 3D mammospheres in suspension culture.

Indeed, when we assessed these cells in suspension culture, overexpression of MELK significantly increased the proliferation of HMEC cells (Figure 4 — figure supplement 1A).

Indeed, we found that MELK overexpression in non-transformed HMEC - DD cells resulted in increased cell proliferation in suspension culture (Figure 4 — figue supplement 2A).