Sentences with phrase «cell assay for»

This workshop will showcase real - time, live - cell assays for immune cell activation, proliferation, chemotaxis, transendothelial migration, immune cell killing, and phagocytosis.

Not exact matches

• NanoCellect Biomedical, a San Diego - based developer of microfluidic cell sorting technologies for cell - based assays, raised $ 10 million in series B funding.

When those cells were placed on a standard wound assay (for measuring properties like cell migration and proliferation), they moved unusually fast.

The system's speed makes it ideal for assay optimization and for assessing cell - based assay quality to reveal cell - seeding errors, improper liquid handling, and bacterial contamination.

Stagljar and his colleagues also applied the new technology, which they dubbed MaMTH (for Mammalian - Membrane Two - Hybrid assay), to identify a protein that plays a role in the most common form of lung cancer called non-small cell lung cancer.

An antibody that works well for Western blots on denaturing gels, for example, should not be presumed to work equally well in nondenaturing assays such as fluorescence - activated cell sorting.

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

«The retraction states that Mignot and his colleagues were unable to replicate the results of the ELISpot assay, a widely used method for measuring how immune system cells such as T cells respond to fragments of foreign proteins, called antigens,» Underwood writes.

Both assays (for translation enhancement and internal ribosomal initiation) were validated under cell - free conditions and in human cells, using a vaccinia virus vector.

They sent soil samples for DNA testing, looking for matches with particular genes known to be found in microbes and fungi; they tried to stimulate microbial growth on a wide variety of substances and then count the cells produced; and they used highly sensitive radiorespiration activity assays, which involve feeding the soil microorganisms a food source which has been labelled with radioactive carbon, which can then be used to detect if the microorganisms are active.

An example of these tools in our lab is the characterization and use of halide - sensitive variants of GFP to screen for activators and inhibitors of CFTR in cell - based assays.

«The basic assays for repeat number, methylation status, and the number of uninterrupted repeats cost less than $ 5, the mark typically considered the threshold for population - based screening,» said Bruce Hayward, PhD, a Senior Research Fellow in the Laboratory of Cell and Molecular Biology and the report's first author.

Oxnard, working with researchers from the Belfer Institute for Applied Cancer Science at Dana - Farber, had recently developed an assay to detect and quantify EGFR mutations in cell - free DNA.

We assayed from 6000 to 20,000 shRNAs simultaneously to identify genes important for the proliferation and survival of five cell lines derived from human mammary tissue.

However, these assays are unsuitable for screening primary cells and can not assess impact on endogenous gene expression.

For Matrigel invasion assay, CSCs were labeled with Cell tracker green (Invitrogen) and 50,000 cells were seeded into Matrigel - coated Transwell insert (Corning) supplemented with DMEM with 10 % serum.

For humanized mice samples, whole genome amplification using the REPLI - g Mini Kit (Qiagen) was conducted prior to the surveyor nuclease assay due to limiting cell numbers.

Cell growth was monitored every 48 hours post-stimulation for approximately two weeks and the efficiency of CXCR4 disruption was assessed at day five post-transduction by both the Surveyor nuclease assay and by deep - sequencing of the CXCR4 target site.

Assays and technologies for circulating cell - free DNA analysis continue to improve and advance, but there is still much to do before these can be used for routine clinical use.

Phenotypic stability and unlimited self - renewal capacity of cell derived from pluripotent stem cells are essential properties for cell assay development for High Throughput Screening (HTS) strategies.

The Molecular Screening and Protein Expression Shared Resource fosters collaboration by providing expertise in biochemical and cell - based assay development for high - throughput screening and compound profiling.

AMSBIO has launched a range of new TIGIT receptor products including a Jurkat cell line, Homogeneous assay kits and recombinant proteins for immunotherapy research

Critical issues for this goal are the number of fetal cells which can be recovered from a blood sample, the purity of cell recovery, the quality of the recovered fetal cells DNA and the assay workflow allowing to develop a high - throughput analysis generating reliable results at a very affordable price.

The level of fluorescence you saw in the validation assay should give an accurate indication of how many pups will contain a mutation and many of the mice with the mutation will be homozygous for it in every cell; however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice.

Fluorescent transgenic ES cell lines - PCR assays are provided for the marker transgene.

The assays presented here demonstrate a rapid and simple system for testing individual and combinations of intrinsic and extrinsic factors for their ability to direct pluripotent cells to multipotent retinal cells, with the option of testing them functionally.

Using an in vitro proxy assay, i.e. the ability of single cells to generate multicellular spheres in suspension (mammosphere assay), it was determined that the mesenchymal subpopulation of HMLE cells is enriched for mammosphere - forming cells.

Members of CRI's Cancer Immunotherapy Consortium (CIC) and the Immunoguiding Program (CIP) of the Germany - based Association for Cancer Immunotherapy (CIMT) announce the completion of the Minimal Information About T cell Assays (MIATA) Project to establish baseline requirements for reporting the results of T cell immune monitoring in clinical trials.

Methods: Human corneas with attached scleral rims were obtained from eye banks and were assayed for telomerase activity and BrdU (bromodeoxyridine) incorporation to determine, respectively, the presence of a stem - like cell marker and replicative activity.

TNA3: BPRC will offer access to its library of human cell reporter assays for the identification of innate immune receptor - induced signalling cascades, including human cell lines transfected with PAMPs such as TLR and / or engineered to express luciferase in response to NF - kB or IFRE - mediated signal transduction, and cell lines that provide insight into the intracellular signalling cascades of innate immune receptors.

Inclusion Criteria: • Eastern Cooperative Oncology Group (ECOG) performance status of 0 or 1 • Have histologically or cytologically confirmed advanced or metastatic non-small cell lung cancer (NSCLC)(Stage IIIb or greater) • Measurable disease, as defined by Response Evaluation Criteria in Solid Tumors (RECIST) 1.1 • Known PD - L1 tumor status as determined by an immunohistochemistry (IHC) assay performed by the central laboratory on tissue obtained at Screening • A woman of childbearing potential must have a negative highly sensitive serum (beta - human chorionic gonadotropin [beta - hCG]-RRB- at Screening within 14 days prior to study drug administration Inclusion Criteria for Crossover: • Participants must have been randomized to Arm A of the study and had radiographic disease progression according to RECIST 1.1 • Participants must have a mandatory biopsy at the time of disease progression according to RECIST 1.1 prior to crossing over.

Rabbit corneas were also assayed for BrdU incorporation to compare their evidence of cell division with that of humans.

Lloyd Old, Thierry Boon, and colleagues develop the TNF release assay for mouse systems in which release of TNF by T cells could be used to assess specific T cell recognition, facilitating the cloning of human tumor antigens.

While mass cytometry offers a powerful and highly multiplexed method for profiling single cells, there are various technical considerations for researchers wanting to perform a mass cytometry assay for the first time, or at a more advanced level.

A collaborative group including the CRI Cancer Immunotherapy Consortium (CIC), the Association for Cancer Immunotherapy (CIMT) Immunoguiding Program (CIP), and scientists at Stanford University launch the Minimal Information About T cell Assays (MIATA) Project, the first systematic effort to establish a framework for reporting immune monitoring data from T cell assays used in clinical trials, with the goal of fostering reliability of data to facilitate meaningful interpretation, comparison, and meta - analysis across clinical tAssays (MIATA) Project, the first systematic effort to establish a framework for reporting immune monitoring data from T cell assays used in clinical trials, with the goal of fostering reliability of data to facilitate meaningful interpretation, comparison, and meta - analysis across clinical tassays used in clinical trials, with the goal of fostering reliability of data to facilitate meaningful interpretation, comparison, and meta - analysis across clinical trials.

Spleen cells were isolated 7 — 14 days after C57BL / 6 CD4 KO mice had rejected BALB / c corneal allografts and were tested for anti - BALB / c CTL in a conventional 4 - hr 51Cr - release assay using BALB / c corneal epithelial and endothelial target cells.

Human primary hepatocytes and immortalized cell lines (e.g., HepaRG) are used to study these disorders, but due to these cells» high variability, low availability, short assay window, or lack of physiological relevance, researchers studying metabolic diseases are searching for more robust and reliable models.

In HeLa CD4 + cell assay this virus has an IC50 value for ddI and ddC around 6 - 10 fold that of the wild type parental virus HXB2.

To check for the presence of this subpopulation of SP cells, we exposed HCT116 to increasing concentrations of Bleomycin and performed Hoechst efflux assay.

The Clinical Biomarkers Facility offers services for high - throughput and highly specific analyses of protein biomarker candidates in body fluids such as plasma, serum, cerebrospinal fluids etc. and cell and tissue lysates using molecular tools such as proximity extension and proximity ligation technologies (PEA and PLA) providing assays with high specificity and sensitivity in complex biological matrices.

In bench assays using living nerve cells to monitor new neuronal connections, Harding, Wright and their colleagues found Dihexa to be seven orders of magnitude more powerful than BDNF, which has yet to be effectively developed for therapeutic use.

In HeLa CD4 + cell assay this virus has an IC50 value for 3TC and FTC around 1000 fold that of the wild type parental virus HXB2.

His laboratory (The Laboratory for Cell and Gene Medicine) specializes in the development of manufacturing processes and QC assays and provides cGMP compliant clinical materials production and regulatory support activities for investigational cell produCell and Gene Medicine) specializes in the development of manufacturing processes and QC assays and provides cGMP compliant clinical materials production and regulatory support activities for investigational cell producell products.

In HeLa CD4 + cell assay this virus has an IC50 value for AZT around 60 - 70 fold that of the wild type parental virus HXB2.

Contribute to the hands - on development and performance of immune - monitoring assays for patients receiving candidate cell therapies

Here we show how second messengers relevant to the Gq signaling pathway can be detected in living cells using assays for DAG, PIP2, and Ca2 +.

Professor Wahl and his team have developed a platform cell - based assay in high - throughput format to screen for inhibitors and agonists of protein - protein interactions.

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

For the colony formation assay, cells were trypsinized to generate single - cell suspensions and counted by a hemocytometer.

Positive data for blood brain barrier (BBB) penetration (High) were also obtained for J147 using the MDCK - MDRI cell culture model for assaying the BBB penetration potential of drugs.