Sentences with phrase «cell colonies with»

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The democratic societies have no one supreme or dominant member, with examples being such things possibly as stones and probably as some cell - colonies and even special forms of many - celled plants and animals.42 Monarchic societies, on the other hand, do have a supreme or dominant member which radically subordinates the parts to its ruling purpose but which can never completely rob the parts of all measure of control over themselves.

By dosing all the post-transplant bacteria with tetracycline and looking for blue colonies, the scientists could identify which cells had the donor DNA.

They showed in previous studies with cell cultures that when adding RK - 33 to malignant lung and other cancerous cells that highly express DDX3, proliferation slowed or halted, and the cells» ability to form colonies was impaired.

To remedy that absence, Golden's lab, along with plant physiologist Takao Kondo and colleagues at Nagoya University in Japan, developed an easy - to - read gauge of changing photosynthetic activity in colonies of the cyanobacterium Synechococcus, a blue - green alga whose one - celled organisms divide as often as once every 5 to 6 hours.

It turns out that even asexual fungal cells can be social: As they grow and fuse with one another to form colonies, they reach out toward some neighbors while snubbing others.

They also filled the tablet with GMC - SF (granulocyte macrophage colony - stimulating factor), an inflammatory protein known to attract immune cells.

The stressed yeast cells lost or duplicated random chromosomes when they divided, producing colonies with a vast array of freak cells.

«The organization of cell colonies and phenotypic switching between different types of colonies becomes a lot more flexible and rapid with reversible aneuploidy than if it depended on random mutations in the genes,» Skupin says.

The yeast Saccharomyces cerevisiae, which normally occurs as a single cell, has the ability to form colonies featuring multicellular structures with divided responsibilities, meaning the cells differentiate to perform different tasks.

Finally, the researchers discovered that ROS generation subsided as the iPSCs matured, and these mature stem cell colonies survive best in a cellular environment with low levels of ROS.

Colonies of genetically corrected cells taken from Fanconi anemia patients show red and yellow, markers associated with pluripotency.

Joseph Poole, M.D., Ph.D., of the Emory University School of Medicine, Atlanta, and colleagues studied whether therapy with granulocyte - macrophage colony stimulating factor (GM - CSF), an agent that functions as a white blood cell growth factor, would improve walking performance in patients with symptomatic peripheral artery disease (a form of vascular disease in which there is partial or total blockage of an artery, usually one leading to a leg or arm).

A huge colony of receptors must be optimally positioned and functioning on our muscle cells for our brains to talk with our bodies so we can walk and breathe.

Plerixafor has been approved by the FDA as the first small - molecule CXCR4 antagonist for use in combination with granulocyte - colony stimulating factor (GCSF) to mobilize hematopoietic stem cells to the bloodstream for collection and subsequent autologous transplantation in patients with non-Hodgkin's lymphoma and multiple myeloma.

Plerixafor in combination with granulocyte - colony stimulating factor (G - CSF) has been approved for hematopoietic stem cell mobilization to the peripheral blood in patients with non-Hodgkin's lymphoma and multiple myeloma [54].

For the colony formation assay, HuH - 7, HeLa, HCT116, and NIH 3T3 cells were transfected with pcDNA - SRα / FLAG - DDX 3 or its parental empty vector by the calcium phosphate method or by Effectene (Qiagen, Hilden, Germany).

After only 10 days — instead of the more typical three to four weeks — one out of 100 hundred cells grew into a tiny colony with all the markings of a typical human embryonic stem cell colony.

Thereafter, sample file was send to the company with Cell - IQ images of the colonies where the analysis protocol was built and tested for correct area recognition.

Analysis by FACS of the pooled HS237 colonies (n = 143), with a total of 3.0 million cells at day 7, revealed that an average size colony of 1.04 mm2 contained ~ 21000 cells (n = 29).

The combination of intermediate - dose cyclophosphamide (ID - CTX) and granulocyte colony - stimulating factor (G - CSF) fails to mobilize peripheral blood stem cells (PBSCs) in approximately 20 % of treated patients with multiple myeloma (MM).

The iPSC colonies were morphologically indistinguishable from hESCs, forming tight colonies of cells with a large nucleus to cytoplasm ratio and prominent nucleoli (Fig. 2E), and they did not display the NSCs» original cell morphology (Fig. 2F).

We also observed that single cells from either the V+S + or V − S + fractions were both equally effective at generating clonal cultures with the normal range of Venus expression and in no cases did V+S + cells give rise to differentiated colonies.

While this manuscript was in preparation, a similar episomal strategy was used to reprogram human primary fibroblasts using a distinct pluripotent cocktail of factors, validating our methods, but with significant lower efficiency (only 3 to 6 colonies / 106 input cells)[42].

Suddenly cells change the kind of food they eat, change their resistance to antibiotics and even grow colonies with completely different shapes.

Interestingly, we detected several colonies when cells were transfected with Oct4 alone.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

A single co-transfection using two and four Cas9 / gRNAs enabled isolation of cell colonies harboring double and quadruple disruptions in 20 % and 2 % of colonies, respectively, which may not be easily achieved with ZFNs and TALENs technologies.

E, Established human iPSC colonies, with well - defined borders and compact cells, are morphologically similar to hESCs.

At this point, some colonies seemed unstable, with a strong tendency to spontaneously differentiate and form a heterogeneous population of cells (Fig. 2B, C).

To produce EBs, ES cell colonies were removed from feeder cells using a detaching solution, which consisted of 0.25 % trypsin supplemented with 1 mg / ml collagenase IV, 1 mM CaCl2, and 20 % knockout serum replacement (KSR; Invitrogen, Carlsbad, CA).

As seen in cells dissected from colonies, significant numbers of cells coexpressed lineage specific markers along with pluripotency genes.

We sought first to examine gene expression in single ES cells isolated directly from specific zones within colonies, with two objectives.

When we further characterized the cell lines derived from the iPSC - like colonies, we observed that they possessed hESC - like morphology, growing as flat colonies with large nucleo - cytoplasmic ratios, defined borders and prominent nucleoli (Figure 4C).

We observed that all three biological replicates with the transduced SSEA3 - negative cells formed many large background colonies (10 — 27 per replicate, Figure 4A) but no iPSC colonies emerged; in contrast, all three biological replicates with the transduced SSEA3 - positive cells resulted in the formation of iPSC colonies (4 — 5 per replicate, Figure 4B) but very few large background colonies (0 — 1 per replicate, Table 1).

Growth factor therapy with granulocyte colony stimulating factor (G - CSF)-- to stimulate the bone marrow to make more white blood cells

(B) Percent of isolated single cells expressing stem or lineage markers according to location with the colony.

Cells towards the edge of the colony are in intimate contact with the feeder cell layer, which produces stem cell maintenance factors such as the BMP antagonist gremlin [21], GDF - 11, and GDF - 8 [34].

The transfected cells were selected with puromycin and subjected to a colony formation assay.

Caffeinated coffee increased plasma levels of granulocyte - colony stimulating factor (GCSF), which seemed to improve the cognitive performance of AD transgenic mice with the recruitment of bone marrow cells, enhanced synaptogenesis, and increased neurogenesis.

The Minnesota team also found that hemangiosarcoma cells with a high surface expression of a protein called colony - stimulating factor 1 receptor (CSF - 1R) were the culprits in this process.

Treatment with chemotherapy may reduce the ability of the circulating cancer cells to establish viable colonies in the lungs.

These are the games whose control schemes I studied to come up with the perfect scheme: Ace Combat 6, Colony Wars, Star Wars Rogue Squadron, SDF Macross (PS2), Metal Gear Solid 4, Splinter Cell Conviction, Grand Theft Auto IV, Vanquish, A.C.E. 3, Anubis, Border Break, Modern Warfare, Gears of War, Dead Space, Orbiter (PC), Shattered Horizon, DropShip, Lost Planet & Transformers: War for Cybertron.

«Corals live in a symbiotic relationship with zooxanthellae, which are plant - like cells hosted in surface tissues that provide up to 90 % of the energy to the colony,» said Stephen Simpson, a marine biologist at the University of Exeter in the UK, commenting on the study.

They can form colonies in which they work together to manipulate our cell surfaces with their retracting appendages until they're allowed entry inside, where they can surreptitiously multiply.