Sentences with phrase «cell surface expression of»

The prominent cell surface expression of CD123 makes this antigen well suited for antibody - drug conjugate (ADC)- based therapeutic strategies.

There is controversy surrounding the cell surface expression of MHC alloantigens by MSC.

Not exact matches

Investigators linked the ability of regulatory T cells to dampen the Th2 response in part to the ability of LKB1 to restrain expression of the cell surface receptor PD - 1 and possibly other receptors.

In their report published in Science Immunology they describe how expression of a specific molecule — complement C5a — is required to cause the immune cells called neutrophils to adhere to joint surfaces and migrate into the joint, a process known to set off the inflammatory cascade.

The good news about the complex interconnectivity is that the scientists also were able to find a potential future point of intervention: When they gave a drug that increased AMPA receptor expression on the cell surface, the spines assumed a more healthy, mature state.

Researchers from BUSM and the University of Cyprus compared the markers on the surface of the cancer cells to gene expression profile of breast tumors deposited by researchers in international public databases and found that a molecule named IL13RA2 (IL13R alpha2) was abundant in metastatic or late - stage BLBC.

Questions the group hopes to answer over the next five years include if LRAs will promote the expression of viral protein on the surface of infected cells, and if pairing LRAs with immune interventions will lead to the clearance of persistent, latent infection.

In particular, Nguyen and Ehrenstein discovered that adalimumab increases the expression of TNF on the surface of patient monocytes and promotes the association of these TNF molecules with receptor proteins on the surface of regulatory T cells.

The researchers speculate that the act of reprogramming adult cells to pluripotency may induce the expression of cell - surface molecules the immune system has not seen since the animal (or person) was an early embryo.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

These findings suggest that animal cells may have fail - safe mechanisms that prevent the surface expression of improperly folded proteins with unpaired or improperly bonded cysteine residues.

For example, the researchers were able to identify previously unknown gene expression differences between the neural stem cells that give rise to the brain's deep structures versus its neocortical surface, and to show that molecular signatures of different neural cell types arise much earlier in brain development than previously realized.

F3 can detect the expression of a protein called nucleolin, which is a marker on the surface of tumor cells.

«By eliminating the expression of the IgE receptor on the surface of mast cells, we have identified an innovative and targeted approach with the potential to treat allergic inflammation in millions of patients worldwide.

As expected, all of the endothelial cell lines showed diminished constitutive expression of E-selectin (data not shown) and VCAM - 1 (Fig. 4C) ⇓ in comparison with surface levels of ICAM - 1 (Fig. 4B) ⇓.

Established cell lines exhibited several inherent endothelial properties, including the expression of constitutive and inducible levels of endothelial cell adhesion molecules E-selectin, intercellular adhesion molecule - 1, and vascular cell adhesion molecule - 1, internalization of acetylated low - density lipoprotein, and formation of loop structures on Matrigel surfaces.

Incomplete restoration of colony stimulating factor - 1 (CSF - 1) function in CSF - 1 — deficient Csf1op / Csf1op mice by transgenic expression of cell surface CSF - 1.

We also noted constitutive surface expression of the tyrosine kinase receptors Tie - 2, FGFR - 1, and Flk - 1 on proliferating and resting endothelial cells (data not shown).

We will develop mathematical models to predict the pathways of differentiation from naive to memory and effector T - cell subsets based on the characterisation of surface marker expression, transcription factors and cytokines production at early and late time points after immunisation.

The expression of the proprotein convertase furin is strongly associated with increased metastasis (migration) of cancer cells; this is thought to be due to its ability to activate the cell surface enzymes responsible for extracellular matrix breakdown.

High level of cell surface CD4 expression.

To compare T cell phenotypes between DGKζ − / − and Cbl - b − / − mice, and to evaluate DKO mice, we isolated thymi, lymph nodes, and spleens from mice of each genotype and determined expression of T cell surface markers.

For instance we know only of very few factors that specifically regulate the expression, the activity and the localisation of K2P channels at the cell surface.

Differential expression of surface markers in mouse bone marrow mesenchymal stromal cell subpopulations with distinct lineage commitment.

Ig - independent Igβ expression on the surface of B lymphocytes after B cell receptor aggregation.

Roles of RabGEF1 / Rabex -5 domains in regulating FcϵRI surface expression and FcϵRI - dependent responses in mast cells.

Lineage - restricted progenitors are identifiable by expression of distinct combinations of cell surface markers.

Human MSCs are defined by positive expression of the cell surface antigens CD73, CD90, CD105 and by absence of expression of hematopoietic antigens, including CD11b or CD14, CD34, CD45, CD79 or CD19, and HLA - DR.

Susceptibility to tuberculosis is associated with TLR1 polymorphisms resulting in a lack of TLR1 cell surface expression.

This allows for the identification, evaluation, and isolation of stem and progenitor cells based on aldehyde dehydrogenase (ALDH), expression and not their cell surface phenotype.

Paul Insel, MD, professor of pharmacology and medicine, will investigate the expression of the GPCR family of receptors on the surface of cells from patients with chronic lymphocytic leukemia (CLL).

ES cells can be described based on a characteristic morphology, the presence of cell surface markers such as SSEA - 1 and Pecam1, or the expression of the key transcription factors such as Oct4, Sox2, Nanog, and a number of ES cell - specific transcripts (ECATs)[4]--[6].

These compartments of cells can be resolved on the basis of their expression of cell surface antigens.

In the present study, we again observed a continuous gradient in the expression of pluripotency genes across the cell populations that paralleled the gradient in cell surface marker expression.

(B) Percent of sorted single HES3 cells in (A) expressing stem or lineage markers according to level of surface marker expression.

Reduced expression of the Indy (= I'm Not Dead Yet) gene, which encodes a cell surface transporter for tri - and dicarboxylic acids, prolongs life and health span in a manner akin to caloric restriction in D. melanogaster and C. elegans.

We derived a fibroblast line from a skin biopsy from a healthy adult male (HUF1)(Figure 1A) and used immunocytochemistry to characterize the expression of cell surface markers commonly expressed on pluripotent stem cells (Figure 1B, C and D).

Our laboratory used two monoclonal antibodies, GCTM - 2 and TG30, recognizing the cell surface proteoglycan characteristic of primate ES cells and CD9 respectively, to fractionate human ES cell populations into four compartments according to their relative levels of expression of both markers [14].

This gradient of surface antigen expression was paralleled by a gradient in expression of pluripotency genes, with highest levels of genes like Oct - 4 found in the population of cells with the highest level of antigen expression.

A. Fractionation of HES 3 or H9 cells by flow cytometry according to the levels of expression of cell surface markers (GCTM - 2, pericellular matrix proteoglycan, and CD9).

Cells along the continuum show a progressively decreasing likelihood of self renewal as their expression of stem cell surface markers and pluripotency genes wanes.

Single cell Q - RTPCR for the genes indicated was carried out on cells separated by flow cytometry on the basis of cell surface marker expression.

Flow cytometry allows researchers to quantify and isolate rare viable cell subsets from heterogenous populations in single - cell suspensions based on differential expression of surface antigens.

Oct - 4 is most consistently expressed of the pluripotency genes that we have studied, and it is switched off only in populations that have lost other measurable features of pluripotency, such as stem cell surface marker expression and the capacity for self - renewal.

Heterogeneity in human ES cultures is reflected by the variability in expression of cell surface antigens seen under culture conditions that promote stem cell renewal.

The pathways that connect expression of stem cell surface glycoconjugates such as the TRA -1-60 / GCTM - 2 antigen, receptors, and growth factors in human and even mouse ES cells with the transcriptional networks that regulate pluripotency remain unclear.

Levels of expression of pluripotency genes declined in parallel with the levels of stem cell surface antigens.

Unbiased clustering analysis of P (+) neurons revealed four different classes, each with distinct cell surface receptor gene expression profiles.

This loss of binding is often accompanied by a loss of ability to express (Barnwell et al. 1983; Handunetti et al. 1987; Gilks et al. 1990)-- or a major alteration in the level of expression of (David et al. 1983; Fandeur et al. 1995)-- the highly variable and clonally variant switching parasite antigens on the surface of the red cell known to be important for the maintenance of long - term chronic infections (Brown and Brown 1965).

Intestinal permeability was assessed by Ussing chamber; epithelial cell (EC) ultra-structure by electron microscopy; RNA expression of genes coding for junctional proteins by Q - real - time PCR; immune response by in - vitro antigen - specific T - cell proliferation and cytokine analysis by cytometric bead array; intestinal microbiota by fluorescence in situ hybridization and analysis of systemic antibodies against intestinal microbiota by surface staining of live bacteria with serum followed by FACS analysis.