Sentences with phrase «cells use rna»

Ultimately, cells use this RNA message as instructions for constructing a protein — the huntingtin protein.

At various points in this process, cells use RNA as a sort of scaffold to help replicate DNA.

Modern cells use RNA to make proteins, the workhorses of cellular function, but RNA likely preceded both proteins and DNA.

Not exact matches

Antisense drugs are essentially pieces of DNA that bind to specific RNAs — the recipe that cells use to make proteins.

The team confirmed that genetic «knockdown» of PRMT1 significantly impaired PDAC cell growth in vitro through use of genetic editing tools, including CRISPR and small hairpin RNA (shRNA).

The team designed DNA circuits that simultaneously test for 24 distinct regions of viral RNA — more than would have been possible using only naturally occurring DNA sequences (Cell, doi.org/wn4).

By using engineered zinc - finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one - cell rat embryo leads to a high frequency of animals carrying 25 to 100 % disruption at the target locus.

They all use RNA molecules as messengers to transfer the information from DNA to cellular factories called ribosomes, which then build proteins, which in turn drive our metabolisms and form the structures of our cells.

The researchers used the dead guide RNAs to turn on the Pdx gene in the mice's livers, which caused the liver cells to produce insulin, reversing the mice's diabetes.

«Controlling RNA in living cells: Modular, programmable proteins can be used to track or manipulate gene expression.»

To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single - cell level, gene expression in samples from six early - stage human tumors.

Using a technique known as single - cell RNA sequencing, the team explored more than 65,000 individual cells that exist under normal or inflammatory conditions, looking for genes that were more active in one state or subpopulation versus another.

The cell uses small RNAs, known as microRNAs, to accomplish the task.

When the team used fragments of interfering RNA to sabotage the production of beta - catenin in these stem cells, the blood cells returned to an early leukaemic state.

Using fluorescence inside living cells as well as biochemistry, they showed that SMN promotes an interaction between the «zipcode» region of a test RNA and a transport protein.

His laboratory discovered that some of the same RNA that is inside human cells are also present in saliva and can be used to detect diseases — a surprising finding, he said, because enzymes in saliva can degrade RNA, making the mouth «a hostile environment.»

The viruses also exploit an enzyme that cells use to destroy RNA to instead produce short stretches of RNA that, among other things, may help the virus avoid the immune system of its host.

Plants grown from these cells make abnormal versions of messenger RNA, the molecule used to make the proteins.

Researchers have discovered that, like plants and invertebrate animals, mammals use the RNA interference (RNAi) process to destroy viruses within their own cells.

Cells in most animals and plants use short - interfering RNA (siRNA) segments to inhibit the formation of viral proteins; here siRNA designed to target IAPV would be fed to colonies as part of double - stranded RNA mixed into a syrup.

Quintana - Murci and colleagues used RNA - sequencing to characterize the way that immune cells, known as primary monocytes, derived from 200 people of self - reported African or European ancestry would respond to attack by a bacteria or a virus.

First, it is a quality control mechanism used by cells to eliminate faulty messenger RNA (mRNA)-- molecules that are essential for transcribing genetic information into the construction of proteins critical for life.

Using a powerful genetic technology called RNA - seq, these experiments revealed a nearly comprehensive list of all the genes, including some not previously identified, that guide the development of taste cells.

DNA - In CRISPR has been specially formulated to enable highly efficient transfection of large plasmids [containing CRISPR - associated protein 9 (Cas9), guide RNAs, and reporter cassettes], particularly when using hard - totransfect cell types.

Hiromi Imamichi, Ph.D., and colleagues used a technique for creating multiple copies of nearly full - length proviral DNA and cell - associated HIV RNA.

In order to locate all gene switches, the Freiburg research team used modern sequencing methods to examine the entire genome — DNA, epigenetic markers and RNA — during the development, maturation and disease of human cardiac muscle cells.

CRISPR is an enzyme that scientists have been able to «program» using targeting RNA in order to cut DNA at precise locations that the cell then repairs on its own.

Instead, the cell is used as an isolation compartment for its own RNA.

The therapy uses a designer ribozyme, a short strand of RNA that chops up other RNA, to seek and destroy mutant RNA before it can be used to build a protein that kills the eye's rod cells.

Reported in Nature Methods today, the new open source computer tool called Single Cell Consensus Clustering (SC3) was shown to be more accurate and robust than existing methods of analysing single - cell RNA sequence data, and is freely available for researchers to Cell Consensus Clustering (SC3) was shown to be more accurate and robust than existing methods of analysing single - cell RNA sequence data, and is freely available for researchers to cell RNA sequence data, and is freely available for researchers to use.

Using nanoparticles designed and screened for endothelial delivery of short strands of RNA called siRNA, the researchers were able to target RNAi to endothelial cells, which form the linings of most organs.

The SC3 tool was then used to analyse single - cell RNA - sequence data from two patients diagnosed with myeloproliferative neoplasm (MPN) blood cancers.

The researchers tested two anti-CK2 drugs for their ability to stimulate the production of new brown fat in mice: a new small - molecule CK2 - blocker called silmitasertib (CX - 4945), which is already in clinical trials as a cancer therapeutic; and a more precise next - generation antisense oligonucleotide (ASO) drug developed in collaboration with Isis Pharmaceuticals, which eliminates CK2 by blocking the RNA instructions cells use to produce it.

Using single - cell RNA sequencing, the team was able to profile molecular features and metabolic activity of individual beta cells to determine how dividing beta cells differ from non-dividing cells.

Split Pool Ligation - based Transcriptome sequencing (SPLiT - seq) is a scalable technique for characterizing RNA in individual cells that can be used to identify the various cell types found in the brain and other tissues.

The team also studied what happened when PAK4 was removed from the cells, using an RNA silencing technology that can prevent production of specific proteins.

Duax also noted that the results raise questions about some aspects of a hypothesis on the origins of life, called the RNA world, which posits that RNA, which is similar to DNA and is still used in cells, was the first genetic material.

The use of cell surface markers to isolate specific cell populations is one common method for separating cells; however, isolating live cells based on their RNA expression is a powerful new way enabling the study of small cell niches in nongenetically modified animal models and human tissue.

The complete structure allows researchers to understand how the polymerase uses host cell RNA (red) to kick - start the production of viral messenger RNA.

HCV invades cells in the body by binding to specific receptors on the cell, enabling the virus to enter it.2 Once inside, HCV hijacks functions of the cell known as transcription, translation and replication, which enables HCV to make copies of its viral genome and proteins, allowing the virus to spread to other sites of the body.2 When HCV enters the host cell, it releases viral (+) RNA that is transcribed by viral RNA replicase into viral -LRB--) RNA, which can be used as a template for viral genome replication to produce more (+) RNA or for viral protein synthesis.

Now, Salk Institute scientists have created a new tool that targets not DNA, but RNA, and used it to correct a protein imbalance in cells from a dementia patient, restoring them to healthy levels.

«In mammals, in the absence of haploid cells, other approaches have been used to identify key genes, such as interfering RNA, but they are sub-optimal methods.

Microbial pathogens induce specific host responses or «RNA biosignatures» that can be identified using microarray analyses of blood leukocytes (white cells).

Doing the screen involves using short pieces of RNA, called small hairpin RNAs, which are inserted into the cell and are able to halt messages from specific genes, keeping the genes from making proteins.

Using sensitive genetic sequencing technology, the scientists identified unique RNA splicing variants that distinguish normal, aging stem cells from abnormal, malignant ones.

The finding was surprising because ZBP1 was known to sense foreign DNA in the cell, but the influenza virus uses RNA as its genetic material.

Furthermore, when they blocked the action of the gene using RNA interference, the cell proliferation index was reduced.

Using experiments with fruit fly eggs, the team saw that Oskar binds to RNA within the cell — specifically three RNAs derived from genes also known to be important to germline development.

«We're talking about messenger RNA (mRNA), which is used by the cell to synthesize these proteins.»

PARTICLE acts in three different ways to prevent expression of the MAT2A gene: 1) by winding around the MAT2A gene to create a DNA: RNA triple helix structure locking down the MAT2A gene promoter, 2) by binding the messenger RNA product of the MAT2A gene and preventing it being used for MAT2A protein synthesis and 3) transferring MAT2A messenger RNA into intracellular vesicles that are subsequently ejected from the cell.